Agouti-related peptide 2 facilitates convergent evolution of stripe patterns across cichlid fish radiations

被引:117
作者
Kratochwil, Claudius F. [1 ,2 ,3 ]
Liang, Yipeng [1 ]
Gerwin, Jan [1 ,3 ]
Woltering, Joost M. [1 ]
Urban, Sabine [1 ,3 ]
Henning, Frederico [1 ,4 ]
Machado-Schiaffino, Gonzalo [1 ,5 ]
Hulsey, C. Darrin [1 ,3 ]
Meyer, Axel [1 ,3 ]
机构
[1] Univ Konstanz, Dept Biol, Constance, Germany
[2] Univ Konstanz, Zukunftskolleg, Constance, Germany
[3] IMPRS OB, Max Planck Inst Ornithol, Constance, Germany
[4] Univ Fed Rio de Janeiro, Fed Univ Rio De Janeiro, Inst Biol, Dept Genet, Rio de Janeiro, Brazil
[5] Univ Oviedo, Dept Funct Biol, Area Genet, Oviedo, Spain
基金
瑞士国家科学基金会; 欧洲研究理事会;
关键词
ADAPTIVE RADIATION; PIGMENTATION; MODEL;
D O I
10.1126/science.aao6809
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The color patterns of African cichlid fishes provide notable examples of phenotypic convergence. Across the more than 1200 East African rift lake species, melanic horizontal stripes have evolved numerous times. We discovered that regulatory changes of the gene agouti-related peptide 2 (agrp2) act as molecular switches controlling this evolutionarily labile phenotype. Reduced agrp2 expression is convergently associated with the presence of stripe patterns across species flocks. However, cis-regulatory mutations are not predictive of stripes across radiations, suggesting independent regulatory mechanisms. Genetic mapping confirms the link between the agrp2 locus and stripe patterns. The crucial role of agrp2 is further supported by a CRISPR-Cas9 knockout that reconstitutes stripes in a nonstriped cichlid. Thus, we unveil how a single gene affects the convergent evolution of a complex color pattern.
引用
收藏
页码:457 / +
页数:4
相关论文
共 24 条
[1]   The genomic substrate for adaptive radiation in African cichlid fish [J].
Brawand, David ;
Wagner, Catherine E. ;
Li, Yang I. ;
Malinsky, Milan ;
Keller, Irene ;
Fan, Shaohua ;
Simakov, Oleg ;
Ng, Alvin Y. ;
Lim, Zhi Wei ;
Bezault, Etienne ;
Turner-Maier, Jason ;
Johnson, Jeremy ;
Alcazar, Rosa ;
Noh, Hyun Ji ;
Russell, Pamela ;
Aken, Bronwen ;
Alfoeldi, Jessica ;
Amemiya, Chris ;
Azzouzi, Naoual ;
Baroiller, Jean-Francois ;
Barloy-Hubler, Frederique ;
Berlin, Aaron ;
Bloomquist, Ryan ;
Carleton, Karen L. ;
Conte, Matthew A. ;
D'Cotta, Helena ;
Eshel, Orly ;
Gaffney, Leslie ;
Galibert, Francis ;
Gante, Hugo F. ;
Gnerre, Sante ;
Greuter, Lucie ;
Guyon, Richard ;
Haddad, Natalie S. ;
Haerty, Wilfried ;
Harris, Rayna M. ;
Hofmann, Hans A. ;
Hourlier, Thibaut ;
Hulata, Gideon ;
Jaffe, David B. ;
Lara, Marcia ;
Lee, Alison P. ;
MacCallum, Iain ;
Mwaiko, Salome ;
Nikaido, Masato ;
Nishihara, Hidenori ;
Ozouf-Costaz, Catherine ;
Penman, David J. ;
Przybylski, Dariusz ;
Rakotomanga, Michaelle .
NATURE, 2014, 513 (7518) :375-+
[2]   Fish pigmentation and the melanocortin system [J].
Cal, Laura ;
Suarez-Bregua, Paula ;
Miguel Cerda-Reverter, Jose ;
Braasch, Ingo ;
Rotllant, Josep .
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, 2017, 211 :26-33
[3]   Pigment patterns in adult fish result from superimposition of two largely independent pigmentation mechanisms [J].
Ceinos, Rosa M. ;
Guillot, Raul ;
Kelsh, Robert N. ;
Cerda-Reverter, Jose M. ;
Rotllant, Josep .
PIGMENT CELL & MELANOMA RESEARCH, 2015, 28 (02) :196-209
[4]  
Gould Gould SJ SJ, Wonderful Life: The Burgess Shale and the Nature of History
[5]   Genetic mapping of horizontal stripes in Lake Victoria cichlid fishes: benefits and pitfalls of using RAD markers for dense linkage mapping [J].
Henning, Frederico ;
Lee, Hyuk Je ;
Franchini, Paolo ;
Meyer, Axel .
MOLECULAR ECOLOGY, 2014, 23 (21) :5224-5240
[6]   Adaptive evolution and explosive speciation: The cichlid fish model [J].
Kocher, TD .
NATURE REVIEWS GENETICS, 2004, 5 (04) :288-298
[7]  
Kratochwil C. F., 2018, DRYAD, DOI [10.5061/dryad.h1107nd, DOI 10.5061/DRYAD.H1107ND]
[8]   Evolution: Tinkering within Gene Regulatory Landscapes [J].
Kratochwil, Claudius F. ;
Meyer, Axel .
CURRENT BIOLOGY, 2015, 25 (07) :R285-R288
[9]   Closing the genotype-phenotype gap: Emerging technologies for evolutionary genetics in ecological model vertebrate systems [J].
Kratochwil, Claudius F. ;
Meyer, Axel .
BIOESSAYS, 2015, 37 (02) :213-226
[10]   Genomic analysis of cichlid fish 'natural mutants' [J].
Kuraku, Shigehiro ;
Meyer, Axel .
CURRENT OPINION IN GENETICS & DEVELOPMENT, 2008, 18 (06) :551-558