Quantitation of porcine cytomegalovirus in pig tissues by PCR

被引：35
作者：
Fryer, JFL
Griffiths, PD
Fishman, JA
Emery, VC
Clark, DA
机构：
[1] UCL, Royal Free & Univ Coll Med Sch, Dept Virol, London NW3 2PF, England
[2] Harvard Univ, Massachusetts Gen Hosp, Sch Med, Transplant Infect Dis Program, Boston, MA USA
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 2001年 / 39卷 / 03期
关键词：
D O I：
10.1128/JCM.39.3.1155-1156.2001
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A quantitative-competitive PCR for the quantification of porcine cytomegalovirus (PCMV) was developed. The virus was detected in a variety of pig organs (including potential xenotransplant donations),,vith viral loads ranging from <10 to: 97 genome copies/<mu>g of DNA. This assay will have significant utility for studying the activation and replication of PCMV and in swine models for allo- and xenotransplantation.
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收藏
页码：1155 / 1156
页数：2
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