Physiochemical changes mediated by "Candidatus Liberibacter asiaticus" in Asian citrus psyllids

被引：12

作者：

Molki, Banafsheh ^{[1
]}

Phuc Thi Ha ^{[1
]}

Mohamed, Abdelrhman ^{[1
]}

Killiny, Nabil ^{[2
]}

Gang, David R. ^{[3
]}

Omsland, Anders ^{[4
]}

Beyenal, Haluk ^{[1
]}

机构：

[1] Washington State Univ, Gene & Linda Voiland Sch Chem Engn & Bioengn, Pullman, WA 99164 USA

[2] Univ Florida, IFAS, Citrus Res & Educ Ctr, Dept Plant Pathol, Lake Alfred, FL USA

[3] Washington State Univ, Inst Biol Chem, Pullman, WA 99164 USA

[4] Washington State Univ, Paul G Allen Sch Global Anim Hlth, Pullman, WA 99164 USA

来源：

SCIENTIFIC REPORTS | 2019年 / 9卷 / 1期

基金：

美国食品与农业研究所;

关键词：

DIAPHORINA-CITRI; PCR DETECTION; SWEET ORANGE; VECTOR; DISEASE; TRANSMISSION;

D O I：

10.1038/s41598-019-52692-7

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Plant pathogenic bacteria interact with their insect host(s)/vector(s) at the cellular and molecular levels. This interaction may alter the physiology of their insect vector, which may also promote the growth and transmission of the bacterium. Here we studied the effect of "Candidatus Liberibacter asiaticus" ("Ca. L. asiaticus") on physiochemical conditions within its insect vector, the Asian citrus psyllid (ACP), and whether these changes were beneficial for the pathogen. The local microenvironments inside ACPs were quantified using microelectrodes. The average hemolymph pH was significantly higher in infected ACPs (8.13 +/- 0.21) than in "Ca. L. asiaticus"-free ACPs (7.29 +/- 0.15). The average hemolymph oxygen tension was higher in "Ca. L. asiaticus"-free ACPs than in infected ACPs (67.13% +/- 2.11% vs. 35.61% +/- 1.26%). Oxygen tension reduction and pH increase were accompanied by "Ca. L. asiaticus" infection. Thus, oxygen tension of the hemolymph is an indicator of infection status, with pH affected by the severity of the infection.

引用

页数：9

共 30 条

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