Polysaccharide from Ostrea rivularis attenuates reproductive oxidative stress damage via activating Keap1-Nrf2/ARE pathway

被引：59
作者：
Li, Shijie [1 ,2 ]
Song, Zhuoyue [1 ,2 ]
Liu, Tingting [4 ]
Liang, Jian [1 ,2 ]
Yuan, Jun [3 ]
Xu, Zhanchi [1 ,2 ]
Sun, Zhanghua [1 ,2 ]
Lai, Xiaoping [1 ,2 ]
Xiong, Qingping [1 ,2 ]
Zhang, Danyan [1 ,2 ]
机构：
[1] Guangzhou Univ Chinese Med, Math Engn Acad Chinese Med, Guangzhou 510006, Guangdong, Peoples R China
[2] Guangzhou Univ Chinese Med, Sch Pharmaceut Sci, Guangzhou 510006, Guangdong, Peoples R China
[3] Huaiyin Inst Technol, Jiangsu Key Lab Reg Resource Exploitat & Med Res, Huaian 223003, Jiangsu, Peoples R China
[4] Xuzhou Med Univ, Affiliated Huaian Hosp, Huaian 223001, Jiangsu, Peoples R China
来源：
CARBOHYDRATE POLYMERS | 2018年 / 186卷
基金：
中国国家自然科学基金;
关键词：
Polysaccharide; Ostrea rivularis; Reproductive oxidative stress damage; Keap1-Nrf2/ARE pathway; SPERM QUALITY; EXPRESSION; ANTIOXIDANT; NRF2; CYCLOPHOSPHAMIDE; SPERMATOGENESIS; CELLS;
D O I：
10.1016/j.carbpol.2018.01.075
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
The purpose of this study was to investigate the effects of Ostrea rivularis polysaccharide (ORP) against testicular oxidative stress injury via kelch-like ECH-associated protein 1-nuclear erythroid 2-related factor 2/antioxidant response element (Keap1-Nrf2/ARE) pathway. In pharmacological experiments in vivo, ORP administration could dose-dependently inhibit body and testicular weight loss, ameliorate epididymal sperm quality and protect reproductive impairment in cyclophosphamide-induced male Balb/c mice. Moreover, the mechanism in vivo might be elucidated that ORP could increase expression level of Nrf2 and its downstream ARE gene battery in the testis, promote production of corresponding antioxidative enzymes and protein, and enhance Keap1-Nrf2/ARE signaling pathway to avoid male reproductive dysfunction. In addition, ORP treatment could improve survival capacity of H2O2-induced TM4 cells and its antioxidant mechanism in vitro also had been verified to activate Keap1-Nrf2/ARE signaling pathway. Overall, these results showed that ORP as a potent antioxidant could reduce reproductive oxidative stress damage related to Keap1-Nrf2/ARE pathway.
引用
收藏
页码：321 / 331
页数：11
相关论文
共 34 条
[1]   Effect of Oxidative Stress on Male Reproduction [J].
Agarwal, Ashok ;
Virk, Gurpriya ;
Ong, Chloe ;
du Plessis, Stefan S. .
WORLD JOURNAL OF MENS HEALTH, 2014, 32 (01) :1-17
[2]   The effects of vitamin E or lipoic acid supplementation on oxyphytosterols in subjects with elevated oxidative stress: a randomized trial [J].
Baumgartner, Sabine ;
Mensink, Ronald P. ;
Haenen, Guido R. ;
Bast, Aalt ;
Binder, Christoph J. ;
Bekers, Otto ;
Husche, Constanze ;
Luetjohann, Dieter ;
Plat, Jogchum .
SCIENTIFIC REPORTS, 2017, 7
[3]   Seipin deficiency increases chromocenter fragmentation and disrupts acrosome formation leading to male infertility [J].
El Zowalaty, A. E. ;
Baumann, C. ;
Li, R. ;
Chen, W. ;
De La Fuente, R. ;
Ye, X. .
CELL DEATH & DISEASE, 2015, 6 :e1817-e1817
[4]   Antioxidant responses and cellular adjustments to oxidative stress [J].
Espinosa-Diez, Cristina ;
Miguel, Veronica ;
Mennerich, Daniela ;
Kietzmann, Thomas ;
Sanchez-Perez, Patricia ;
Cadenas, Susana ;
Lamas, Santiago .
REDOX BIOLOGY, 2015, 6 :183-197
[5]   Injectable Fullerenol/Alginate Hydrogel for Suppression of Oxidative Stress Damage in Brown Adipose-Derived Stem Cells and Cardiac Repair [J].
Hao, Tong ;
Li, Junjie ;
Yao, Fanglian ;
Dong, Dianyu ;
Wang, Yan ;
Yang, Boguang ;
Wang, Changyong .
ACS NANO, 2017, 11 (06) :5474-5488
[6]   Cyclophosphamide and acrolein induced oxidative stress leading to deterioration of metaphase II mouse oocyte quality [J].
Jeelani, Roohi ;
Khan, Sana N. ;
Shaeib, Faten ;
Kohan-Ghadr, Hamid-Reza ;
Aldhaheri, Sarah R. ;
Najafi, Tohid ;
Thakur, Mili ;
Morris, Robert ;
Abu-Soud, Husam M. .
FREE RADICAL BIOLOGY AND MEDICINE, 2017, 110 :11-18
[7]  
Khushboo M, 2017, ENVIRON SCI POLLUT R, V1, P1
[8]   Increased expression of Nrf2/ARE-dependent anti-oxidant proteins in tamoxifen-resistant breast cancer cells [J].
Kim, Sang Kyum ;
Yang, Jin Won ;
Kim, Mi Ra ;
Roh, Sang Hee ;
Kim, Hyung Gyun ;
Lee, Kwang Youl ;
Jeong, Hye Gwang ;
Kang, Keon Wook .
FREE RADICAL BIOLOGY AND MEDICINE, 2008, 45 (04) :537-546
[9]  
Kobayashi A, 2004, METHOD ENZYMOL, V378, P273
[10]   Pectinase-treated Panax ginseng protects against chronic intermittent heat stress-induced testicular damage by modulating hormonal and spermatogenesis-related molecular expression in rats [J].
Kopalli, Spandana Rajendra ;
Cha, Kyu-Min ;
Lee, Sang-Ho ;
Ryu, Ji-Hoon ;
Hwang, Seock-Yeon ;
Jeong, Min-Sik ;
Sung, Jong-Hwan ;
Kim, Si-Kwan .
JOURNAL OF GINSENG RESEARCH, 2017, 41 (04) :578-588
1234