Contemporary uses of old folks: the immunomodulatory and toxic potential of fenbufen

被引:5
作者
Akhtar, Muhammad Furqan [1 ]
Mehr-un-Nisa [1 ]
Saleem, Ammara [2 ]
Hamid, Irfan [3 ]
Alsharif, Khalaf F. [4 ]
Abdel-Daim, Mohamed M. [5 ,6 ]
Rahman, Md Habibur [7 ,8 ]
Shah, Muddaser [9 ]
Sohail, Kashif [10 ]
Javaid, Zeeshan [11 ]
机构
[1] Riphah Int Univ, Riphah Inst Pharmaceut Sci, Lahore Campus, Lahore, Pakistan
[2] Govt Coll Univ Faisalabad, Fac Pharmaceut Sci, Dept Pharmacol, Faisalabad, Pakistan
[3] Univ Punjab, Cadson Coll Pharm, Kharian, Pakistan
[4] Taif Univ, Coll Appl Med Sci, Dept Clin Lab Sci, POB 11099, At Taif 21944, Saudi Arabia
[5] Batterjee Med Coll, Dept Pharmaceut Sci, Pharm Program, POB 6231, Jeddah 21442, Saudi Arabia
[6] Suez Canal Univ, Fac Vet Med, Pharmacol Dept, Ismailia 41522, Egypt
[7] Southeast Univ, Dept Pharm, Dhaka 1213, Bangladesh
[8] Yonsei Univ, Wonju Coll Med, Dept Global Med Sci, Wonju 26426, Gangwon Do, South Korea
[9] Abdul Wali Khan Univ Mardan, Dept Bot, Mardan 23200, Pakistan
[10] Mirpur Univ Sci & Technol, Akson Coll Hlth Sci, Mirpur, Ajk, Pakistan
[11] Mirpur Univ Sci & Technol, Dept Pharm, Mirpur, Ajk, Pakistan
关键词
Anti-inflammatory; Fenbufen; Oxidative stress; Cell-mediated immunity; Humoral immunity; Inflammation; OXIDATIVE STRESS; EXTRACT; ACID;
D O I
10.14715/cmb/2021.67.5.4
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Fenbfen is used for pain, pyrexia and in the management of osteoarthritis, rheumatoid arthritis and other musculoskeletal disorders. The present research was planned to examine the immunomodulatory activity of fenbufen in different models of cell-mediated immunity (CMI) and humoral immunity (HI). The CMI was evaluated by delayed-type hypersensitivity (DTH) and cyclophosphamide-induced neutropenia assays while HI was appraised by hemagglutination (HA) assay by administering fenbufen at 2, 6 and 10 mg.kg(-1) and azathioprine 40 mg.kg(-1) (as standard therapy) to albino mice by intraperitoneal route. The ex vivo immunomodulatory action was determined by red blood cell (RBC) membrane stabilization and protein denaturation assays. The results showed that fenbufen treatment had significantly (p<0.05-p<0.001) reduced white blood cells, hemoglobin content, and red blood cells in the healthy and neutropenic mice. A significant (p<0.001) reduction in activities of superoxide dismutase and catalase and glutathione contents, and enhancement of malondialdehyde level were observed in neutropenic mice that were restored by fenbufen treatment. It suppressed DTH reaction after 24, 48 and 72 h post topical application of 2, 4-dinitrofluorobenzene (DNFB). Fenbufen or azathioprine treated groups also showed a significant reduction in the antibody titer against human RBCs induced immune activation in mice as compared to the disease control mice. Fenbufen showed IC50 of 14.0, 50.5 and 66.2 mu g.ml(-1) whereas, diclofenac sodium showed IC50 of 61.0, 126 and 50.5 mu g/ml in RBCs membrane stabilization, egg albumin and bovine serum albumin denaturation assays respectively. The current study shows that fenbufen might have potential immunomodulatory activity against CMI and HI. It can be utilized to treat immune system disorders. Copyright: (C) 2021 by the C.M.B. Association. All rights reserved.
引用
收藏
页码:27 / 36
页数:10
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