The feasibility of hyperbaric storage (HS) to substitute refrigeration as a lower energetic cost alternative to refrigeration, for sliced cooked ham preservation was assessed by using temperatures and pressures ranging 25-37 degrees C and 25-150 MPa for 4 and 8 h. At microbiological level, storage at 25 degrees C, 30 degrees C, and 37 degrees C, showed no effect on microbial growth at 25 MPa reaching levels similar to atmospheric pressure storage, around 5 log CFU/g for both total aerobic mesophiles (TAM) and lactic acid bacteria (LAB). Nevertheless, the storage at 50 MPa and 30 degrees C resulted in microbial growth inhibition, resulting in TAM and LAB counts similar to refrigeration, of about 3.8 log CFU/g for both the microorganisms. Additionally, the increase of the storage pressure to 100-150 MPa resulted in microbial inactivation, leading to microbial loads of almost 1 log CFU/g lower than refrigeration. In general, hyperbaric stored sliced cooked ham showed physicochemical parameters similar to the refrigerated samples. In conclusion, these results show that HS at uncontrolled (naturally variable room temperature conditions at 25-37 degrees C) is a promising alternative to refrigeration for cooked ham preservation. To this new preservation technology, no energetic costs are associated throughout storage, compared to refrigeration, needing only energy to generate the pressure and decompress, since no energy is required to maintain the pressure. (C) 2015 The Institution of Chemical Engineers. Published by Elsevier B.V. All rights reserved.