Quantifying transport dynamics with three-dimensional single-particle tracking in adherent cells

被引：5
|
作者：
Jiang, Chao [1 ,2 ,3 ,4 ]
Dou, Shuo-Xing [3 ,4 ]
Wang, Peng-Ye [3 ,4 ,5 ]
Li, Hui [1 ,2 ,6 ]
机构：
[1] Beijing Normal Univ, Sch Syst Sci, Beijing 100875, Peoples R China
[2] Beijing Normal Univ, Inst Nonequilibrium Syst, Beijing 100875, Peoples R China
[3] Chinese Acad Sci, Beijing Natl Lab Condensed Matter Phys, Beijing 100190, Peoples R China
[4] Chinese Acad Sci, Inst Phys, Lab Soft Matter Phys, Beijing 100190, Peoples R China
[5] Univ Chinese Acad Sci, Sch Phys Sci, Beijing 100049, Peoples R China
[6] Songshan Lake Mat Lab, Dongguan 523808, Guangdong, Peoples R China
来源：
STAR PROTOCOLS | 2022年 / 3卷 / 04期
基金：
中国国家自然科学基金;
关键词：
QUANTUM-DOT;
D O I：
10.1016/j.xpro.2022.101790
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Intracellular transport plays an important role in maintaining the physiological functions of cells. Here, we describe a protocol for 3D single-particle tracking within living cells. We detail the use of a two-focal imaging system and the analytical steps for quantifying 3D transport dynamics. This protocol can be used to characterize the intracellular diffusion and trafficking of macromolecules, nano particles, and endocytic vesicles in adherent cells. For complete details on the use and execution of this protocol, please refer to Jiang et al. (2022).
引用
收藏
页数：20
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