Peroxidase-catalyzed co-oxidation of 3,3′,5,5′-tetramethylbenzidine in the presence of substituted phenols and their polydisulfides

The steady-state kinetics of the horseradish peroxidase (HRP)-catalyzed oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) has been studied in the presence of 2-amino-4-nitrophenol (ANP), gallic acid (GA) or 4,4-dihydroxydiphenylsulfone (DDS) and their polydisulfides poly(ADSNP), poly(DSGA), poly(DSDDS) at 20 degreesC in 10 mM phosphate buffer, pH 6.4, supplemented with 5-10% dimethylformamide. The second-order rate constants for the reactions of ANP, GA, poly(DSGA) and poly(DSDDS) with HRP-. Compound I (k(2)) and Compound 11 (k(3)) have been determined at 25 degreesC in 10 mM phosphate buffer, pH 6.0 by stopped-flow spectrophotometry. ANP, GA and their polydisulfides strongly inhibited HRP-catalyzed TMB oxidation. Inhibition constants (K-i) and stoichiometric coefficients of inhibition (f) have been determined for these reactions. The most effective inhibitor was poly (DSGA) (K-i = 1.3 muM, f = 35.6). The oxidation of substrate pairs by HRP, i.e., TMB-DDS and TMB-poly(DSDDS) at pH 7.2 resulted in a similar to8- and similar to12-fold stimulation of TMB oxidation rates, respectively. The mechanisms of the HRP-catalyzed co-oxidation of TMB-phenol pairs are discussed. (C) 2003 Elsevier Inc. All rights reserved.