Circular dichroism to determine binding mode and affinity of ligand-DNA interactions

被引：275
|
作者：
Garbett, Nichola C. [1 ]
Ragazzon, Patricia A. [1 ]
Chaires, Jonathan B. [1 ]
机构：
[1] Univ Louisville, James Graham Brown Canc Ctr, Louisville, KY 40202 USA
来源：
NATURE PROTOCOLS | 2007年 / 2卷 / 12期
关键词：
D O I：
10.1038/nprot.2007.475
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Circular dichroism (CD) is a useful technique for an assessment of DNA-binding mode, being a more accessible, low-resolution complement to NMR and X-ray diffraction methods. Ligand-DNA interactions can be studied by virtue of the interpretation of induced ligand CD signals resulting from the coupling of electric transition moments of the ligand and DNA bases within the asymmetric DNA environment. This protocol outlines methods to determine the binding mode and affinity of ligand-DNA interactions and takes approximately 7.5 h.
引用
收藏
页码：3166 / 3172
页数：7
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