Cryopreservation of sterlet (Acipenser ruthenus) spermatozoa using different cryoprotectants

被引：28
作者：
Boryshpolets, S. [1 ]
Dzyuba, B. [1 ,2 ]
Rodina, M. [1 ]
Alavi, S. M. H. [1 ]
Gela, D. [1 ]
Linhart, O. [1 ]
机构：
[1] Univ S Bohemia, Fac Fisheries & Protect Water, Res Inst Fish Culture & Hydrobiol, Vodnany 38925, Czech Republic
[2] Natl Acad Sci Ukraine, Inst Problems Cryobiol & Cryomed, Kharkov, Ukraine
来源：
JOURNAL OF APPLIED ICHTHYOLOGY | 2011年 / 27卷 / 05期
关键词：
SPERM CRYOPRESERVATION; STURGEON; MOTILITY; METHANOL; BIOLOGY;
D O I：
10.1111/j.1439-0426.2011.01866.x
中图分类号：
S9 [水产、渔业];
学科分类号：
0908 ;
摘要：
The present study examined the effectiveness of different cryoprotectan uses for cryopreservation of sterlet (Acipenser ruthenus) sperm. Four different cryoprotectans [dimethylsulfoxide (DMSO), dimethylacetamide (DMA), ethyleneglycol (EG) and methanol (MET)] in concentrations of 5 and 10% in the extender media (30 mM sucrose, 1 mM KCl, 25 mM Tris-HCl pH 8.5) were used for the cryopreservation of sperm from five sterlet males. Percentages of motility, velocity, fertilization and hatching rate were measured. The highest post-thawing motility was observed in sperm samples cryopreserved with 10% MET (46 +/- 19%), 10% DMA (47 +/- 18%) and 10% DMSO (45 +/- 7%). Fertilization rate in the control (fresh sperm) was 69 +/- 9% and the hatching rate 61 +/- 8%. A higher hatching rate after cryopreservation was obtained with 10% MET (32 +/- 17%) and 5% DMA (23 +/- 3%) when compared to data obtained with 10% DMA (9 +/- 5%), 5% MET and 5 and 10% DMSO, wherein the hatching rate was around zero. Our results demonstrate that DMA can be used for cryopreservation of sturgeon spermatozoa.
引用
收藏
页码：1147 / 1149
页数：3
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