Effects of high hydrostatic pressure on secondary structure and emulsifying behavior of sweet potato protein

被引:20
作者
Khan, Nasir Mehmood [1 ,2 ]
Mu, Tai-Hua [1 ]
Sun, Hong-Nan [1 ]
Zhang, Miao [1 ]
Chen, Jing-Wang [1 ]
机构
[1] Chinese Acad Agr Sci, Inst Agroprod Proc Sci & Technol, Minist Agr, Key Lab Agroprod Proc, Beijing 100193, Peoples R China
[2] Shaheed Benazir Bhutto Univ, Dept Chem, Dir Upper 18000, KP, Pakistan
关键词
sweet potato protein; emulsion; circular dichroism; high hydrostatic pressure; DROPLET SIZE; ISOLATE; PH;
D O I
10.1080/08957959.2015.1005013
中图分类号
O4 [物理学];
学科分类号
0702 ;
摘要
In this study, secondary structures of sweet potato protein (SPP) after high hydrostatic pressure (HHP) treatment (200-600 MPa) were evaluated and emulsifying properties of emulsions with HHP-treated SPP solutions in different pH values (3, 6, and 9) were investigated. Circular dichroism analysis confirmed the modification of the SPP secondary structure. Surface hydrophobicity increased at pH 3 and decreased at 6 and 9. Emulsifying activity index at pH 6 increased with an increase in pressure, whereas emulsifying stability index increased at pH 6 and 9. Oil droplet sizes decreased, while volume frequency distribution of the smaller droplets increased at pH 3 and 6 with the HHP treatment. Emulsion viscosity increased at pH 6 and 9 and pseudo-plastic flow behaviors were not altered for all emulsions produced with HHP-treated SPP. These results suggested that HHP could modify the SPP structure for better emulsifying properties, which could increase the use of SPP emulsion in the food industry.
引用
收藏
页码:189 / 202
页数:14
相关论文
共 30 条
[1]   STRUCTURE-FUNCTION-RELATIONSHIPS OF COWPEA (VIGNA-UNGUICULATA) GLOBULIN ISOLATE - INFLUENCE OF PH AND NACL ON PHYSICOCHEMICAL AND FUNCTIONAL-PROPERTIES [J].
ALUKO, RE ;
YADA, RY .
FOOD CHEMISTRY, 1995, 53 (03) :259-265
[2]  
[Anonymous], 2005, FOOD EMULSIONS PRINC
[3]  
[Anonymous], ROOTS TUB PLANT BAN
[4]  
Association of Official Analytical Chemists (AOAC), 1990, METHODS ANAL
[5]  
Bovell-Benjamin AC, 2007, ADV FOOD NUTR RES, V52, P1, DOI 10.1016/S1043-4526(06)52001-7
[6]   DETERMINATION OF INTERFACIAL-AREAS IN EMULSIONS USING TURBIDIMETRIC AND DROPLET SIZE DATA - CORRECTION OF THE FORMULA FOR EMULSIFYING ACTIVITY INDEX [J].
CAMERON, DR ;
WEBER, ME ;
IDZIAK, ES ;
NEUFELD, RJ ;
COOPER, DG .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1991, 39 (04) :655-659
[7]   Improvement of emulsifying properties of lupin proteins by high pressure induced aggregation [J].
Chapleau, N ;
de Lamballerie-Anton, M .
FOOD HYDROCOLLOIDS, 2003, 17 (03) :273-280
[8]   Interfacial properties of deamidated wheat protein in relation to its ability to stabilise oil-in-water emulsions [J].
Day, Li ;
Xu, Mi ;
Lundin, Leif ;
Wooster, Tim J. .
FOOD HYDROCOLLOIDS, 2009, 23 (08) :2158-2167
[9]   Effect of high pressure on the emulsifying behaviour of beta-lactoglobulin [J].
Galazka, VB ;
Dickinson, E ;
Ledward, DA .
FOOD HYDROCOLLOIDS, 1996, 10 (02) :213-219
[10]   High pressure effects on emulsifying behavior of whey protein concentrate [J].
Galazka, VB ;
Ledward, DA ;
Dickinson, E ;
Langley, KR .
JOURNAL OF FOOD SCIENCE, 1995, 60 (06) :1341-1343