Biosynthesis of silver nanoparticle (AgNPs) using Lactobacillus and their effects on oxidative stress biomarkers in rats

Biosynthesis method of nanoparticles acquires very important area due to their economic and ecofriendly benefits. The present study was aimed to the biosynthesis of silver nanoparticles (AgNPs) using Lactobacillus mixture and evaluating their antioxidant activity. The characterization and biosynthesis AgNPs was achieved, using Ultra Violet (UV)-Visible spectrophotometry. Scanning electron microscope (SEM) was used to detect the size, shape and distribution of AgNPs. The occurrence of elemental silver was analyzed by Energy Dispersive-X-ray Spectroscopy (EDS) analysis. To evaluate the antioxidant activity of AgNPs and Lactobacillus in vivo, forty healthy adult rats were used and divided into eight equal groups, first group served as control, second group received LAB mix1 (1 ml/kg) and three groups were administrated with three concentration of AgNPs (5, 50 and 500 mg/kg AgNPs) respectively and other three groups were administrated with the same concentration of AgNPs along with LAB mix1 for two weeks. The results revealed significant increased (p < 0.05) in total antioxidant capacity (TAC) of (LAB mix1 1 ml/kg, 5 mg/kg AgNPs, 5 mg/kg AgNPs + LAB mix1, 50 mg/kg AgNPs + LAB mix1 and 500 mg/kg AgNPs + LAB mix1) and significantly decreased (p < 0.05) in treatments of (50 and 500 mg/kg AgNPs). The current study demonstrated that Lactobacillus afforded beneficial role by increasing the antioxidant activity with AgNPs and as ameliorative function for the effect of high dose of AgNPs. (C) 2017 The Author. Production and hosting by Elsevier B.V. on behalf of King Saud University.