Effects of light stress on the expression of Kisspeptin/GnRH in rat hypothalamus

被引：0
作者：
Luo, Li [1 ]
Wang, Youmin [1 ,2 ]
Deng, Datong [1 ]
Cheng, Yuan [3 ]
Zhou, Huanli [2 ]
机构：
[1] Anhui Med Univ, Affiliated Hosp 1, Inst Endocrinol & Metab, Dept Endocrinol, 218 Jixi Rd, Hefei 230022, Anhui, Peoples R China
[2] Anhui Prov Lab Endocrinol & Metab, Hefei, Anhui, Peoples R China
[3] Anhui Med Univ, Affiliated Hosp 2, Inst Endocrinol & Metab, Dept Endocrinol, Hefei, Anhui, Peoples R China
来源：
INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE | 2016年 / 9卷 / 08期
关键词：
Hypothalamus; kisspeptin; GnRH; chronic stress; GONADOTROPIN-RELEASING-HORMONE; PROTEIN-KINASE-C; SIGNAL-REGULATED KINASE; N-TERMINAL KINASE; SUBUNIT PROMOTER; KISS-1; PEPTIDE; NATURAL LIGAND; CELL-LINE; ACTIVATION; RECEPTOR;
D O I：
暂无
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
Objective: This study aims to explore the effects of light stress on the expression of Kisspeptin/GnRH in rat hypothalamus. Methods: The rat model of chronic stress was established through artificial illumination time. The rats were divided into control group, continuous light group, interval light group and reverse day and night group randomly. The expression levels of Kisspeptin and GnRH in rat hypothalamus were detected by RT-PCR, western-blotting and immunohistochemical methods. Results: The results showed that the expression levels of Kisspeptin and GnRH in rat hypothalamus significantly decreased in continuous light group and reverse day and night group (P<0.05), there was no difference between interval light group and control group (P>0.05). Conclusions: Long time light stress can decrease the expression levels of Kisspeptin and GnRH in rat hypothalamus.
引用
收藏
页码：16964 / 16970
页数：7
相关论文
共 26 条
  • [1] Astin John A, 2004, Adv Mind Body Med, V20, P14
  • [2] Extracellular signal-regulated kinase, Jun N-terminal kinase, p38, and c-Src are involved in gonadotropin-releasing hormone-stimulated activity of the glycoprotein hormone follicle-stimulating hormone β-subunit promoter
    Bonfil, D
    Chuderland, D
    Kraus, S
    Shahbazian, D
    Friedberg, I
    Seger, R
    Naor, Z
    [J]. ENDOCRINOLOGY, 2004, 145 (05) : 2228 - 2244
  • [3] The neuroendocrine physiology of kisspeptin in the human
    Dhillo, Waljit S.
    Murphy, Kevin G.
    Bloom, Stephen R.
    [J]. REVIEWS IN ENDOCRINE & METABOLIC DISORDERS, 2007, 8 (01) : 41 - 46
  • [4] Context influences the interplay of endocrine axes across the day
    Dismukes, Andrew R.
    Shirtcliff, Elizabeth A.
    Hanson, Jamie L.
    Pollak, Seth D.
    [J]. DEVELOPMENTAL PSYCHOBIOLOGY, 2015, 57 (06) : 731 - 741
  • [5] MOLECULAR-BIOLOGY OF THE PITUITARY GONADOTROPINS
    GHARIB, SD
    WIERMAN, ME
    SHUPNIK, MA
    CHIN, WW
    [J]. ENDOCRINE REVIEWS, 1990, 11 (01) : 177 - 199
  • [6] Developmental Changes in GnRH Release in Response to Kisspeptin Agonist and Antagonist in Female Rhesus Monkeys (Macaca mulatta): Implication for the Mechanism of Puberty
    Guerriero, Kathryn A.
    Keen, Kim L.
    Millar, Robert P.
    Terasawa, Ei
    [J]. ENDOCRINOLOGY, 2012, 153 (02) : 825 - 836
  • [7] Down-Regulation of Hypothalamic Kisspeptin and its Receptor, Kiss1r, mRNA Expression is Associated with Stress-Induced Suppression of Luteinising Hormone Secretion in the Female Rat
    Kinsey-Jones, J. S.
    Li, X. F.
    Knox, A. M. I.
    Wilkinson, E. S.
    Zhu, X. L.
    Chaudhary, A. A.
    Milligan, S. R.
    Lightman, S. L.
    O'Byrne, K. T.
    [J]. JOURNAL OF NEUROENDOCRINOLOGY, 2009, 21 (01) : 20 - 29
  • [8] Selective translocation of non-conventional protein kinase C isoenzymes by gonadotropin-releasing hormone (GnRH) in the gonadotrope-derived alpha T3-1 cell line
    Kratzmeier, M
    Poch, A
    Mukhopadhyay, AK
    McArdle, CA
    [J]. MOLECULAR AND CELLULAR ENDOCRINOLOGY, 1996, 118 (1-2) : 103 - 111
  • [9] In Utero Development of Kisspeptin/GnRH Neural Circuitry in Male Mice
    Kumar, Devesh
    Periasamy, Vinod
    Freese, Maria
    Voigt, Anja
    Boehm, Ulrich
    [J]. ENDOCRINOLOGY, 2015, 156 (09) : 3084 - 3090
  • [10] Stimulation of jun N-terminal kinase (JNK) by gonadotropin-releasing hormone in pituitary αT3-1 cell line is mediated by protein kinase C, c-Src, and CDC42
    Levi, NL
    Hanoch, T
    Benard, O
    Rozenblat, M
    Harris, D
    Reiss, N
    Naor, Z
    Seger, R
    [J]. MOLECULAR ENDOCRINOLOGY, 1998, 12 (06) : 815 - 824
123