Multi-scale engineering for neuronal cell growth and differentiation

被引：21

作者：

Beduer, Amelie ^{[1
,2
,6
]}

Vaysse, Laurence ^{[3
,4
]}

Flahaut, Emmanuel ^{[2
,5
]}

Seichepine, Florent ^{[1
,2
,5
]}

Loubinoux, Isabelle ^{[3
,4
]}

Vieu, Christophe ^{[1
,2
,6
]}

机构：

[1] CNRS, LAAS, F-31077 Toulouse, France

[2] Univ Toulouse, UPS, INSA, INP,ISAE,LAAS, F-31059 Toulouse, France

[3] INSERM, UMR 825, F-31059 Toulouse, France

[4] Univ Toulouse, CHU Purpan, UMR 825, UPS, F-31059 Toulouse, France

[5] Univ Toulouse, UPS, INP, Inst Carnot Cirimat, F-31062 Toulouse, France

[6] Ctr Pierre Potier, ITAV, F-31106 Toulouse, France

来源：

MICROELECTRONIC ENGINEERING | 2011年 / 88卷 / 08期

关键词：

Neuronal cells; Microtopography; Nanotopography; Cell patterning; Carbon nanotubes; NEURITE GROWTH;

D O I：

10.1016/j.mee.2010.12.049

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

In this paper we investigate the role of micropatterning and molecular coating for cell culture and differentiation of neuronal cells (Neuro2a cell line) on a polydimethylsiloxane substrate. We investigate arrays of micrometric grooves (line and space) capable to guide neurite along their axis. We demonstrate that pattern dimensions play a major role due to the deformation of the cell occasioned by grooves narrower than typical cell dimension. A technological compromise for optimizing cell density, differentiation rate and neurite alignment has been obtained for 20 mu m wide grooves which is a dimension comparable with the average cell dimension. This topographical engineered pattern combined with double-wall carbon nanotubes coating enabled us to obtain adherent cell densities in the order of 10(4) cells/cm(2) and a differentiation rate close to 100%. (C) 2011 Elsevier BM. All rights reserved.

引用

页码：1668 / 1671

页数：4

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