Genome Rearrangements in Maize Induced by Alternative Transposition of Reversed Ac/Ds Termini

被引:39
作者
Yu, Chuanhe
Zhang, Jianbo
Peterson, Thomas [1 ]
机构
[1] Iowa State Univ, Dept Genet Dev & Cell Biol, Ames, IA 50011 USA
基金
美国国家科学基金会;
关键词
TRANSPOSABLE-ELEMENT; INSERTIONAL MUTAGENESIS; CHROMOSOME BREAKAGE; TAGGING SYSTEM; DNA-SEQUENCE; DS ELEMENT; GENE; DELETIONS; MODULATOR; PATTERN;
D O I
10.1534/genetics.111.126847
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Alternative transposition can induce genome rearrangements, including deletions, inverted duplications, inversions, and translocations. To investigate the types and frequency of the rearrangements elicited by a pair of reversed Ac/Ds termini, we isolated and analyzed 100 new mutant alleles derived from two parental alleles that both contain an intact Ac and a fractured Ac (fAc) structure at the maize p1 locus. Mutants were characterized by PCR and sequencing; the results show that nearly 90% (89/100) of the mutant alleles represent structural rearrangements including deletions, inversions, translocations, or rearrangement of the intertransposon sequence (ITS). Among 37 deletions obtained, 20 extend into the external flanking sequences, while 17 delete portions of the intertransposon sequence. Interestingly, one deletion allele that contains only a single nucleotide between the retained Ac and fAc termini is not competent for further alternative transposition events. We propose a new model for the formation of intertransposon deletions through insertion of reversed transposon termini into sister-chromatid sequences. These results document the types and frequencies of genome rearrangements induced by alternative transposition of reversed Ac/Ds termini in maize.
引用
收藏
页码:59 / 67
页数:9
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