β-Lactam resistance development affects binding of penicillin-binding proteins (PBPs) of Clostridium perfringens to the fluorescent penicillin, BOCILLIN FL

Alteration in the binding of bacterial penicillin-binding proteins (PBPs) to beta-lactams is important in the development of drug resistance. The PBPs of wild type Clostridium perfringens ATCC 13124 and three beta-lactam-resistant mutants were compared for the ability to bind to a fluorescent penicillin, BOCILLIN FL. The binding of the high molecular weight protein PBP1, a transpeptidase, to BOCILLIN FL was reduced in all of the resistant strains. In contrast, the binding of BOCILLIN FL to a low molecular weight protein, PBP6, a D-alanyl-D-alanine carboxypeptidase that was more abundant in all three resistant strains, was substantially increased. A competition assay with beta-lactams reduced the binding of all of the PBPs, including PBP6, to BOCILLIN FL. beta-Lactams enhanced transcription of the putative gene for PBP6 in both wild type and resistant strains. This is the first report showing that mutations in a high molecular weight PBP and overexpression of a low molecular weight PBP in resistant C. perfringens strains affected their binding to beta-lactams. Published by Elsevier Ltd.