Bioengineered dental tissues grown in the rat jaw

被引：81

作者：

Duailibi, S. E. ^{[2
]}

Duailibi, M. T. ^{[2
]}

Zhang, W.

Asrican, R. ^{[3
,4
]}

Vacanti, J. P. ^{[5
,6
]}

Yelick, P. C. ^{[1
]}

机构：

[1] Tufts Univ, Div Craniofacial & Mol Genet, Dept Oral & Maxillofacial Pathol, Boston, MA 02111 USA

[2] Univ Fed Sao Paulo, Interdisciplinary Ctr Gene Therapy, CINTERGEN, Dept Plast Surg, Sao Paulo, Brazil

[3] Harvard Univ, Sch Dent Med, Forsyth Inst, Dept Cytokine Biol, Boston, MA 02115 USA

[4] Harvard Univ, Sch Dent Med, Dept Dev Biol, Boston, MA 02115 USA

[5] Massachusetts Gen Hosp, Lab Tissue Engn & Organ Fabricat, Boston, MA 02114 USA

[6] Harvard Univ, Sch Med, Dept Surg, Boston, MA 02115 USA

来源：

JOURNAL OF DENTAL RESEARCH | 2008年 / 87卷 / 08期

关键词：

tooth tissue engineering; dental stem cells; mandibular model;

D O I：

10.1177/154405910808700811

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

Our long-term objective is to develop methods to form, in the jaw, bioengineered replacement teeth that exhibit physical properties and functions similar to those of natural teeth. Our results show that cultured rat tooth bud cells, seeded onto biodegradable scaffolds, implanted into the jaws of adult rat hosts and grown for 12 weeks, formed small, organized, bioengineered tooth crowns, containing dentin, enamel, pulp, and periodontal ligament tissues, similar to identical cell-seeded scaffolds implanted and grown in the omentum. Radiographic, histological, and immunohisto chemical analyses showed that bioengineered teeth consisted of organized dentin, enamel, and pulp tissues. This study advances practical applications for dental tissue engineering by demonstrating that bioengineered tooth tissues can be regenerated at the site of previously lost teeth, and supports the use of tissue engineering strategies in humans, to regenerate previously lost and/or missing teeth. The results presented in this report support the feasibility of bioengineered replacement tooth formation in the jaw.

引用

页码：745 / 750

页数：6

共 36 条

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