Axially-offset differential interference contrast microscopy via polarization wavefront shaping

被引:9
作者
Ding, Changqin [1 ]
Li, Chen [1 ]
Deng, Fengyuan [1 ,2 ]
Simpson, Garth J. [1 ]
机构
[1] Purdue Univ, Dept Chem, 560 Oval Dr, W Lafayette, IN 47907 USA
[2] Boston Univ, Dept Elect & Comp Engn, 8 St Marys St, Boston, MA 02215 USA
基金
美国国家卫生研究院;
关键词
DIFFRACTION PHASE MICROSCOPY; LIQUID-CRYSTAL LENS; REFRACTIVE-INDEX; FIELD;
D O I
10.1364/OE.27.003837
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
Sample-scan phase contrast imaging was demonstrated by producing and coherently recombining light from a pair of axially offset focal planes. Placing a homogeneous medium in one of the two focal planes enables quantitative phase imaging using only common-path optics, recovering absolute phase without halo or oblique-illumination artifacts. Axially offset foci separated by 70 mu m with a 10x objective were produced through polarization wavefront shaping using a matched pair of custom-designed microretarder arrays, compatible with retrofitting into conventional commercial microscopes. Quantitative phase imaging was achieved by two complementary approaches: i) rotation of a half wave plate, and ii) 50 kHz polarization modulation with lock-in amplification for detection. (C) 2019 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
引用
收藏
页码:3837 / 3850
页数:14
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