Phosphorylcholine-conjugated gold-molecular clusters improve signal for Lymph Node NIR-II fluorescence imaging in pre-clinical cancer models

被引：78

作者：

Baghdasaryan, Ani ^{[1
,2
]}

Wang, Feifei ^{[1
,2
]}

Ren, Fuqiang ^{[1
,2
]}

Ma, Zhuoran ^{[1
,2
]}

Li, Jiachen ^{[1
,2
]}

Zhou, Xueting ^{[3
]}

Grigoryan, Lilit ^{[4
]}

Xu, Chun ^{[1
,2
]}

Dai, Hongjie ^{[1
,2
]}

机构：

[1] Stanford Univ, Dept Chem, Stanford, CA 94305 USA

[2] Stanford Univ, Bio X, Stanford, CA 94305 USA

[3] Stanford Univ, Sch Engn, Dept Bioengn, Stanford, CA 94305 USA

[4] Stanford Univ, Sch Med, Dept Microbiol & Immunol, Inst Immunol Transplantat & Infect Dis,Dept Patho, Stanford, CA 94305 USA

来源：

NATURE COMMUNICATIONS | 2022年 / 13卷 / 01期

基金：

瑞士国家科学基金会; 美国国家卫生研究院;

关键词：

INDOCYANINE GREEN; METAL NANOCLUSTERS; CARBON NANOTUBES; BREAST-CANCER; STRATEGIES; RETENTION; BIOPSY; BRAIN;

D O I：

10.1038/s41467-022-33341-6

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Sentinel lymph node imaging and biopsy is important to clinical assessment of cancer metastasis, and novel non-radioactive lymphographic tracers have been actively pursued over the years. Here, we develop gold molecular clusters (Au-25) functionalized by phosphorylcholine (PC) ligands for NIR-II (1000-3000 nm) fluorescence imaging of draining lymph nodes in 4T1 murine breast cancer and CT26 colon cancer tumor mouse models. The Au-phosphorylcholine (Au-PC) probes exhibit 'super-stealth' behavior with little interactions with serum proteins, cells and tissues in vivo, which differs from the indocyanine green (ICG) dye. Subcutaneous injection of Au-PC allows lymph node mapping by NIR-II fluorescence imaging at an optimal time of similar to 0.5 - 1 hour postinjection followed by rapid renal clearance. Preclinical NIR-II fluorescence LN imaging with Au-PC affords high signal to background ratios and high safety and biocompatibility, promising for future clinical translation.

引用

页数：11

共 78 条

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