Production of a monoclonal antibody by ascites, hollow fiber system, and transgenic plants for vaccine production using CB.Hep-1 mAb as a study case

被引：3

作者：

Valdes, Rodolfo ^{[1
]}

Tamayo, Andres ^{[1
]}

Gonzalez, Marcos ^{[1
]}

Padilla, Sigifredo ^{[1
]}

Geada, Deborah ^{[2
]}

Ferro, William ^{[1
]}

Mila, Lorely ^{[3
]}

Gomez, Leonardo ^{[1
]}

Aleman, Rosario ^{[1
]}

Leyva, Alberto

Garcia, Cristina ^{[1
]}

Mendoza, Otto ^{[1
]}

Alvarez, Tatiana ^{[1
]}

Dorta, Lamay ^{[1
]}

Villega, Yanet ^{[1
]}

Cecilia, Dobian ^{[1
]}

Aragon, Hasel ^{[1
]}

Gonzalez, Tatiana

La O, Maylin ^{[4
]}

Lopez, Jorge ^{[4
]}

机构：

[1] Ctr Genet Engn & Biotechnol, Monoclonal Antibody Prod Dept, Havana 10600, Cuba

[2] Inst Tobacco Res, Havana 3500, Cuba

[3] Ctr Genet Engn & Biotechnol, Qual Assurance Direct, Havana 10600, Cuba

[4] Ctr Genet Engn & Biotechnol, Qual Control Direct, Havana 10600, Cuba

来源：

BIOTECHNOLOGY AND BIOPROCESS ENGINEERING | 2012年 / 17卷 / 01期

关键词：

ascites; hollow fiber bioreactor; plantibody; monoclonal antibody; transgenic plants; B SURFACE-ANTIGEN; IN-VITRO PRODUCTION; IMMUNOAFFINITY PURIFICATION; RECOMBINANT PROTEINS; EXPRESSION; BIOREACTORS; CONTAMINANTS; GLYCANS;

D O I：

10.1007/s12257-011-0196-2

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Monoclonal antibody (mAb) production methods (ascites, in vitro technologies, transgenic animals, and dicot or monocot transgenic plants; moss, algae) have been improved since they were first developed in 1975. In this study, we illustrate a summary of a study case in which mice, a hollow fiber system, and tobacco transgenic plants were assessed for the production of mAb for vaccine manufacturing and vaccine production.

引用

页码：145 / 159

页数：15

共 8 条

[1] Production of a monoclonal antibody by ascites, hollow fiber system, and transgenic plants for vaccine production using CB.Hep-1 mAb as a study case
Rodolfo Valdés
Andrés Tamayo
Marcos González
Sigifredo Padilla
Déborah Geada
William Ferro
Lorely Milá
Leonardo Gómez
Rosario Alemán
Alberto Leyva
Cristina García
Otto Mendoza
Tatiana Alvarez
Lamay Dorta
Yanet Villega
Dobián Cecilia
Hasel Aragón
Tatiana González
Maylín La O
Jorge López
Biotechnology and Bioprocess Engineering, 2012, 17 : 145 - 159
[2] CB.Hep-1 hybridoma growth and antibody production using protein-free medium in a hollow fiber bioreactor
Valdés, R
Ibarra, N
González, M
Alvarez, T
García, J
Llambias, R
Pérez, CA
Quintero, O
Fischer, R
CYTOTECHNOLOGY, 2001, 35 (02) : 145 - 154
[3] CB.Hep-1 hybridoma growth and antibody production using protein-free medium in a hollow fiber bioreactor
R. Valdés
N. Ibarra
M. González
T. Alvarez
J. García
R. Llambias
C. A. Pérez
O. Quintero
R. Fischer
Cytotechnology, 2001, 35 : 145 - 154
[4] Evaluation of hollow fiber bioreactors as an alternative to murine ascites production for small scale monoclonal antibody production
Jackson, LR
Trudel, LJ
Fox, JG
Lipman, NS
JOURNAL OF IMMUNOLOGICAL METHODS, 1996, 189 (02) : 217 - 231
[5] Production of a recombinant human monoclonal antibody using a novel hollow fiber bioreactor system
Inoue, Y
Kawamoto, S
Seki, K
Teruya, K
Mochizuki, K
Kato, M
Hashizume, S
Yasumoto, K
Nagashima, A
Nakahashi, H
Suzuki, T
Imai, T
Nomoto, K
Shirahata, S
JOURNAL OF FERMENTATION AND BIOENGINEERING, 1996, 81 (05): : 466 - 469
[6] Use of hollow fiber systems for rapid and direct scale up of antibody production from hybridoma cell lines cultured in CL-1000 flasks using BD Cell MAb medium
Michael J. Gramer
Jodi Maas
Michael M. Lieberman
Cytotechnology, 2003, 42 : 155 - 162
[7] Use of hollow fiber systems for rapid and direct scale up of antibody production from hybridoma cell lines cultured in CL-1000 flasks using BD Cell MAb medium
Gramer, MJ
Maas, J
Lieberman, MM
CYTOTECHNOLOGY, 2003, 42 (03) : 155 - 162
[8] MONITORING OF THE PRODUCTION OF MONOCLONAL-ANTIBODIES BY HYBRIDOMAS .1. LONG-TERM CULTIVATION IN HOLLOW FIBER BIOREACTORS USING SERUM-FREE MEDIUM
VANERP, R
ADORF, M
VANSOMMEREN, APG
SCHONHERR, OT
GRIBNAU, TCJ
JOURNAL OF BIOTECHNOLOGY, 1991, 20 (03) : 235 - 248

← 1 →