An investigation of methyl tert-butyl ether-induced cytotoxicity and protein profile in Chinese hamster ovary cells

被引：6

作者：

Xie, Guangshan ^{[1
,2
,3
,4
]}

Hong, Wen-Xu ^{[1
,2
,3
]}

Zhou, Li ^{[2
]}

Yang, Xifei ^{[2
]}

Huang, Haiyan ^{[2
]}

Wu, Desheng ^{[2
]}

Huang, Xinfeng ^{[2
]}

Zhu, Weiguo ^{[4
]}

Liu, Jianjun ^{[1
,2
]}

机构：

[1] Shenzhen Res Inst Populat & Family Planning, 4009 Xinzhou Rd, Shenzhen 518040, Guangdong, Peoples R China

[2] Shenzhen Ctr Dis Control & Prevent, Med Key Lab Hlth Toxicol Shenzhen, Med Key Lab Guangdong Prov, Key Lab Modern Toxicol Shenzhen, Shenzhen 518055, Guangdong, Peoples R China

[3] Shenzhen Blood Ctr, Shenzhen 518035, Guangdong, Peoples R China

[4] Xiangtan Univ, Dept Chem, Xiangtan 411105, Hunan, Peoples R China

来源：

MOLECULAR MEDICINE REPORTS | 2017年 / 16卷 / 06期

关键词：

methyl tert-butyl ether; Chinese hamster ovary cells; oxidative stress; cytotoxicity; proteomics; HEAT-SHOCK PROTEINS; OXIDATIVE STRESS; IN-VITRO; DIFFERENTIAL EXPRESSION; SPERMATOGENIC CELLS; PRIMARY CULTURES; MTBE; TOXICITY; EXPOSURE; GROWTH;

D O I：

10.3892/mmr.2017.7761

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Methyl tert-butyl ether (MTBE) is widely used as an oxygenating agent in gasoline to reduce harmful emissions. However, previous studies have demonstrated that MTBE is a cytotoxic substance that has harmful effects in vivo and in vitro. Although remarkable progress has been made in elucidating the mechanisms underlying the MTBE-induced reproductive toxicological effect in different cell lines, the precise mechanisms remain far from understood. The present study aimed to evaluate whether mammalian ovary cells were sensitive to MTBE exposure in vitro by assessing cell viability, lactate dehydrogenase (LDH) leakage, malondialdehyde (MDA) content and antioxidant enzyme activities. In addition, the effect of MTBE exposure on differential protein expression profiles was examined by two-dimensional electrophoresis and matrix-assisted laser desorption/ionization-time of flight mass spectrometry. MTBE exposure induced significant effects on cell viability, LDH leakage, plasma membrane damage and the activity of antioxidant enzymes. In the proteomic analysis, 24 proteins were demonstrated to be significantly affected by MTBE exposure. Functional analysis indicated that these proteins were involved in catalytic activity, binding, structural molecule activity, metabolic processes, cellular processes and localization, highlighting the fact that the cytotoxic mechanisms resulting from MTBE exposure are complex and diverse. The altered expression levels of two representative proteins, heat shock protein family A (Hsp70) members 8 and 9, were further confirmed by western blot analysis. The results revealed that MTBE exposure affects protein expression in Chinese hamster ovary cells and that oxidative stress and altered protein levels constitute the mechanisms underlying MTBE-induced cytotoxicity. These findings provided novel insights into the biochemical mechanisms involved in MTBE-induced cytotoxicity in the reproductive system.

引用

页码：8595 / 8604

页数：10