Outbreak of typhoid fever in a non-endemic area: Comparison of three molecular typing methods

被引：5

作者：

Gruner, E

Flepp, M

Gabathuler, U

Thong, KL

Altwegg, M

机构：

[1] UNIV ZURICH,DEPT MED,DIV INFECT DIS & HOSP EPIDEMIOL,CH-8091 ZURICH,SWITZERLAND

[2] MINIST HLTH CANTON ZURICH,CH-8090 ZURICH,SWITZERLAND

[3] UNIV MALAYA,CTR FDN STUDIES SCI,KUALA LUMPUR 50603,MALAYSIA

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 1997年 / 28卷 / 03期

关键词：

pulsed-field gel electrophoresis (PFGE); random amplified polymorphic DNA (RAPD); ribotyping; Salmonella typhi;

D O I：

10.1016/S0167-7012(97)00977-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An outbreak of typhoid fever in Zurich, Switzerland, which involved seven customers and three employees of a city mall restaurant, was investigated by comparing three molecular typing methods: pulsed-field gel electrophoresis (PFGE), ribosomal RNA gene restriction patterns (ribotyping), and random amplified polymorphic DNA (RAPD). Both PFGE and ribotyping identified two molecular patterns among the outbreak-related isolates which differed in one band: these isolates were considered clonally related and differed clearly from other unrelated S. typhi strains. RAPD could not distinguish among outbreak isolates and control strains. (C) 1997 Elsevier Science B.V.

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收藏

页码：179 / 185

页数：7

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