Orexin (hypocretin) gene expression in rat ependymal cells

被引：7
作者：
Kummer, M [1 ]
Neidert, SJ [1 ]
Jöhren, O [1 ]
Dominiak, P [1 ]
机构：
[1] Univ Clin Lubeck, Inst Expt & Clin Pharmacol & Toxicol, D-23538 Lubeck, Germany
来源：
NEUROREPORT | 2001年 / 12卷 / 10期
关键词：
brain ventricle; choroid plexus; ependyma; in situ hybridization; prepro-orexin;
D O I：
10.1097/00001756-200107200-00015
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
The expression of prepro-orexin (PPO) mRNA in the rat brain was investigated by in situ hybridization histochemistry. In the lateral and posterior hypothalamic areas, which are considered to produce exclusively PPO mRNA, we found high levels of PPO mRNA expressions. We also localized PPO mRNA hybridization signals at lower levels around the lateral ventricles, the third and fourth ventricle. Cellular analysis by emulsion autoradiography revealed the expression of PPO mRNA in the ependymal cell layer. Our results demonstrate that beside the lateral and posterior hypothalamus PPO mRNA is expressed in ependymal cells. NeuroReport 12:2117-2120 (C) 2001 Lippincott Williams & Wilkins.
引用
收藏
页码：2117 / 2120
页数：4
相关论文
共 25 条
  • [1] Bruni JE, 1998, MICROSC RES TECHNIQ, V41, P2, DOI 10.1002/(SICI)1097-0029(19980401)41:1<2::AID-JEMT2>3.3.CO
  • [2] 2-T
  • [3] Narcolepsy in orexin knockout mice:: Molecular genetics of sleep regulation
    Chemelli, RM
    Willie, JT
    Sinton, CM
    Elmquist, JK
    Scammell, T
    Lee, C
    Richardson, JA
    Williams, SC
    Xiong, YM
    Kisanuki, Y
    Fitch, TE
    Nakazato, M
    Hammer, RE
    Saper, CB
    Yanagisawa, M
    [J]. CELL, 1999, 98 (04) : 437 - 451
  • [4] Orexin A-like immunoreactivity in the rat brain
    Chen, CT
    Dun, SL
    Kwok, EH
    Dun, NJ
    Chang, JK
    [J]. NEUROSCIENCE LETTERS, 1999, 260 (03) : 161 - 164
  • [5] Differential distribution of orexin-A and orexin-B immunoreactivity in the rat brain and spinal cord
    Cutler, DJ
    Morris, R
    Sheridhar, V
    Wattam, TAK
    Holmes, S
    Patel, S
    Arch, JRS
    Wilson, S
    Buckingham, RE
    Evans, ML
    Leslie, RA
    Williams, G
    [J]. PEPTIDES, 1999, 20 (12) : 1455 - 1470
  • [6] Orexins, orexigenic hypothalamic peptides, interact with autonomic, neuroendocrine and neuroregulatory systems
    Date, Y
    Ueta, Y
    Yamashita, H
    Yamaguchi, H
    Matsukura, S
    Kangawa, K
    Sakurai, T
    Yanagisawa, M
    Nakazato, M
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (02) : 748 - 753
  • [7] The hypocretins: Hypothalamus-specific peptides with neuroexcitatory activity
    De Lecea, L
    Kilduff, TS
    Peyron, C
    Gao, XB
    Foye, PE
    Danielson, PE
    Fukuhara, C
    Battenberg, ELF
    Gautvik, VT
    Bartlett, FS
    Frankel, WN
    van den Pol, AN
    Bloom, FE
    Gautvik, KM
    Sutcliffe, JG
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (01) : 322 - 327
  • [8] THE EPENDYMA - A PROTECTIVE BARRIER BETWEEN BRAIN AND CEREBROSPINAL-FLUID
    DELBIGIO, MR
    [J]. GLIA, 1995, 14 (01) : 1 - 13
  • [9] Orexin A activates locus coeruleus cell firing and increases arousal in the rat
    Hagan, JJ
    Leslie, RA
    Patel, S
    Evans, ML
    Wattam, TA
    Holmes, S
    Benham, CD
    Taylor, SG
    Routledge, C
    Hemmati, P
    Munton, RP
    Ashmeade, TE
    Shah, AS
    Hatcher, JP
    Hatcher, PD
    Jones, DNC
    Smith, MI
    Piper, DC
    Hunter, AJ
    Porter, RA
    Upton, N
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (19) : 10911 - 10916
  • [10] Effect of lateral cerebroventricular injection of the appetite-stimulating neuropeptide, orexin and neuropeptide Y, on the various behavioral activities of rats
    Ida, T
    Nakahara, K
    Katayama, T
    Murakami, N
    Nakazato, M
    [J]. BRAIN RESEARCH, 1999, 821 (02) : 526 - 529
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