INFLUENCE OF LOW-MOLECULAR (BELOW 5 KDA) FRACTION FROM CORD BLOOD AND ACTOVEGIN ON PHAGOCYTIC ACTIVITY OF FROZEN-THAWED NEUTROPHILS

The influence of the cattle cord blood low-molecular fraction (below 5 kDa) as part of the rehabilitating medium in comparison with Actovegin on the functional activity of neutrophils after cryopreservation was studied. Incubation of frozen-thawed neutrophils in the rehabilitating media containing the low-molecular fraction or Actovegin was established to stimulate their phagocytic function, in particular engulfing and digesting ability. It was shown that after incubation of frozen-thawed neutrophils in the media containing CBF at the concentration 0.15 mg/ml or Actovegin at the concentration 1.5 mg/ml their oxygen-dependent metabolism was activated, since the number of NBT-positive neutrophils increased significantly in comparison with the control. Gel-penetrating chromatography of CBF and Actovegin revealed differences between their chromatograms reflecting differences between the compositions compared. Our investigations proved that recovery of the functional activity of frozen-thawed neutrophils was possible in the media containing the cattle cord blood low-molecular fraction (below 5 kDa) or Actovegin at the concentrations 0.15 mg/ml and 1.5 mg/ml, respectively.