Sclerenchyma cell thickening through enhanced lignification induced by OsMYB30 prevents fungal penetration of rice leaves

被引：118

作者：

Li, Weitao ^{[1
,2
]}

Wang, Kang ^{[1
,2
]}

Chern, Mawsheng ^{[3
]}

Liu, Yuchen ^{[1
,2
]}

Zhu, Ziwei ^{[1
,2
]}

Liu, Jiang ^{[1
,2
]}

Zhu, Xiaobo ^{[1
,2
]}

Yin, Junjie ^{[1
,2
]}

Ran, Li ^{[1
,2
]}

Xiong, Jun ^{[1
,2
]}

He, Kaiwei ^{[1
,2
]}

Xu, Liting ^{[1
,2
]}

He, Min ^{[1
,2
]}

Wang, Jing ^{[1
,2
]}

Liu, Jiali ^{[1
,2
]}

Bi, Yu ^{[1
,2
]}

Qing, Hai ^{[1
,2
]}

Li, Mingwu ^{[1
,2
]}

Hu, Kun ^{[1
,2
]}

Song, Li ^{[1
,2
]}

Wang, Long ^{[1
,2
]}

Qi, Tuo ^{[1
,2
]}

Hou, Qingqing ^{[1
,2
]}

Chen, Weilan ^{[1
,2
]}

Li, Yan ^{[1
,2
]}

Wang, Wenming ^{[1
,2
]}

Chen, Xuewei ^{[1
,2
]}

机构：

[1] Sichuan Agr Univ Wenjiang, State Key Lab Crop Gene Explorat & Utilisat South, Key Lab Major Crop Dis, State Key Lab Hybrid Rice,Rice Res Inst, Chengdu 611130, Sichuan, Peoples R China

[2] Sichuan Agr Univ Wenjiang, Collaborat Innovat Ctr Hybrid Rice Yangtze River, Rice Res Inst, Chengdu 611130, Sichuan, Peoples R China

[3] Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USA

来源：

NEW PHYTOLOGIST | 2020年 / 226卷 / 06期

基金：

中国国家自然科学基金; 美国国家卫生研究院; 美国国家科学基金会; 美国食品与农业研究所;

关键词：

broad-spectrum resistance; lignin; Magnaporthe oryzae; penetration; rice; sclerenchyma; TRANSCRIPTION FACTOR; MAGNAPORTHE-GRISEA; DISEASE RESISTANCE; BLAST RESISTANCE; GENE-EXPRESSION; PROTEIN; IMMUNITY; DEFENSE; BIOSYNTHESIS; INFECTION;

D O I：

10.1111/nph.16505

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Broad-spectrum resistance is highly preferred in crop breeding programmes. Previously, we have reported the identification of the broad-spectrum resistance-Digu 1 (bsr-d1) allele from rice Digu. The bsr-d1 allele prevents activation of Bsr-d1 expression by Magnaporthe oryzae infection and degradation of H2O2 by peroxidases, leading to resistance to M. oryzae. However, it remains unknown whether defence pathways other than H2O2 burst and peroxidases contribute to the bsr-d1-mediated immunity. Blast resistance was determined in rice leaves by spray and punch inoculations. Target genes of OsMYB30 were identified by one-hybrid assays in yeast and electrophoretic mobility shift assay. Lignin content was measured by phloroglucinol-HCl staining, and acetyl bromide and thioacidolysis methods. Here, we report the involvement of the OsMYB30 gene in bsr-d1-mediated blast resistance. Expression of OsMYB30 was induced during M. oryzae infection or when Bsr-d1 was knocked out or downregulated, as occurs in bsr-d1 plants upon infection. We further found that OsMYB30 bound to and activated the promoters of 4-coumarate:coenzyme A ligase genes (Os4CL3 and Os4CL5) resulting in accumulation of lignin subunits G and S. This action led to obvious thickening of sclerenchyma cells near the epidermis, inhibiting M. oryzae penetration at the early stage of infection. Our study revealed novel components required for bsr-d1-mediated resistance and penetration-dependent immunity, and advanced our understanding of broad-spectrum disease resistance.

引用

页码：1850 / 1863

页数：14

共 48 条

[1] An OsCEBiP/OsCERK1-OsRacGEF1-OsRac1 Module Is an Essential Early Component of Chitin-Induced Rice Immunity [J].