One-pot production of fructooligosaccharides by a Saccharomyces cerevisiae strain expressing an engineered invertase

被引:18
|
作者
Marin-Navarro, Julia [1 ]
Talens-Perales, David [1 ]
Polaina, Julio [1 ]
机构
[1] CSIC, Inst Agroquim & Tecnol Alimentos, Valencia 46980, Spain
关键词
Cell factory; Kestose; Prebiotics; Sucrose; Transglycosylation; OLIGOSACCHARIDES; YEAST; FERMENTATION; 6-KESTOSE;
D O I
10.1007/s00253-014-6312-4
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
We describe a simple, efficient process for the production of 6-kestose, a trisaccharide with well-documented prebiotic properties. A key factor is the use of a yeast transformant expressing an engineered version of Saccharomyces invertase with enhanced transfructosylating activity. When the yeast transformant was grown with 30 % sucrose as the carbon source, 6-kestose accumulated up to ca. 100 g/L in the culture medium. The 6-kestose yield was significantly enhanced (up to 200 g/L) using a two-stage process carried out in the same flask. In the first stage, the culture was grown in 30 % sucrose at physiological temperature (30 A degrees C) to allow overexpression of the invertase. In the second stage, sucrose was added to the culture at high concentration (60 %) and the temperature shifted to 50 A degrees C. In both cases, 6-kestose was synthesized with high specificity, representing more than 95 % of total FOS.
引用
收藏
页码:2549 / 2555
页数:7
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