The cytoplasmic zinc finger protein ZPR1 accumulates in the nucleolus of proliferating

被引：75
作者：
Galcheva-Gargova, Z
Gangwani, L
Konstantinov, KN
Mikrut, M
Theroux, SJ
Enoch, T
Davis, RJ [1 ]
机构：
[1] Univ Massachusetts, Sch Med, Howard Hughes Med Inst, Worcester, MA 01605 USA
[2] Univ Massachusetts, Sch Med, Dept Biochem & Mol Biol, Program Mol Med, Worcester, MA 01605 USA
[3] Assumption Coll, Dept Biol, Worcester, MA 01615 USA
[4] Scripps Res Inst, Dept Mol & Expt Med, La Jolla, CA 92037 USA
[5] Harvard Univ, Sch Med, Dept Genet, Boston, MA 02115 USA
来源：
MOLECULAR BIOLOGY OF THE CELL | 1998年 / 9卷 / 10期
关键词：
D O I：
10.1091/mbc.9.10.2963
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
The zinc finger protein ZPR1 translocates from the cytoplasm to the nucleus after treatment of cells with mitogens. The function of nuclear ZPR1 has not been defined. Here we demonstrate that ZPR1 accumulates in the nucleolus of proliferating cells. The role of ZPR1 was examined using a gene disruption strategy. Cells lacking ZPR1 are not viable. Biochemical analysis demonstrated that the loss of ZPR1 caused disruption of nucleolar function, including preribosomal RNA expression. These data establish ZPR1 as an essential protein that is required for normal nucleolar function in proliferating cells.
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页码：2963 / 2971
页数：9
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