Adsorption equilibria of proline in hydrophilic interaction chromatography

被引：30

作者：

Vajda, Peter ^{[1
]}

Felinger, Attila ^{[1
]}

Cavazzini, Alberto ^{[2
]}

机构：

[1] Univ Pecs, Dept Analyt & Environm Chem, H-7624 Pecs, Hungary

[2] Univ Ferrara, Dept Chem, I-44100 Ferrara, Italy

来源：

JOURNAL OF CHROMATOGRAPHY A | 2010年 / 1217卷 / 38期

关键词：

Proline; Frontal analysis; HILIC; Equilibrium dispersive model; PHASE LIQUID-CHROMATOGRAPHY; MOBILE-PHASE; COMPONENTS; ISOTHERMS; SURFACE; VOLUME; GASES; WATER;

D O I：

10.1016/j.chroma.2010.07.058

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The adsorption behavior of proline under hydrophilic interaction chromatography conditions was investigated from six aqueous solutions of acetonitrile. Proline adsorption isotherms were recorded at each mobile phase composition by frontal analysis and inverse method. The BET model was found to be the best choice to describe the nonlinear behavior of proline adsorption under hydrophilic interaction chromatography conditions. The adsorption isotherm parameters were derived from two independent parameter estimation methods. The parameters derived from regression analysis of the frontal analysis data and from overloaded elution bands were found to be in good agreement with the excess isotherm of water. The mobile phase composition at which the maximum excess adsorption of water was observed corresponded to the maximum saturation capacity measured for praline. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：5965 / 5970

页数：6

共 20 条

[1] HYDROPHILIC-INTERACTION CHROMATOGRAPHY FOR THE SEPARATION OF PEPTIDES, NUCLEIC-ACIDS AND OTHER POLAR COMPOUNDS [J].

ALPERT, AJ .

JOURNAL OF CHROMATOGRAPHY, 1990, 499 :177-196

[2] On a theory of the van der Waals adsorption of gases [J].

Brunauer, S ;

Deming, LS ;

Deming, WE ;

Teller, E .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1940, 62 :1723-1732

[3] Adsorption of gases in multimolecular layers [J].

Brunauer, S ;

Emmett, PH ;

Teller, E .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1938, 60 :309-319

[4] Interpretation of the excess adsorption isotherms of organic eluent components on the surface of reversed-phase phenyl modified adsorbents [J].

Chan, F ;

Yeung, LS ;

LoBrutto, R ;

Kazakevich, Y .

JOURNAL OF CHROMATOGRAPHY A, 2005, 1082 (02) :158-165

[5] Repeatability and reproducibility of high-concentration data in reversed-phase liquid chromatography -: III.: Isotherm reproducibility on Kromasil C18 [J].

Felinger, A ;

Gritti, F ;

Guiochon, G .

JOURNAL OF CHROMATOGRAPHY A, 2004, 1024 (1-2) :21-38

[6] Numerical determination of the competitive isotherm of enantiomers [J].

Felinger, A ;

Cavazzini, A ;

Guiochon, G .

JOURNAL OF CHROMATOGRAPHY A, 2003, 986 (02) :207-225

[7] Efficiency of the same neat silica column in hydrophilic interaction chromatography and per aqueous liquid chromatography [J].

Gritti, Fabrice ;

Pereira, Alberto dos Santos ;

Sandra, Pat ;

Guiochon, Georges .

JOURNAL OF CHROMATOGRAPHY A, 2010, 1217 (05) :683-688

[8] Comparison of the adsorption mechanisms of pyridine in hydrophilic interaction chromatography and in reversed-phase aqueous liquid chromatography [J].

Gritti, Fabrice ;

Pereira, Alberto dos Santos ;

Sandra, Pat ;

Guiochon, Georges .

JOURNAL OF CHROMATOGRAPHY A, 2009, 1216 (48) :8496-8504

[9]

Guiochon G., 2006, FUNDAMENTALS PREPARA

[10]

Ha N.L., 1982, Anal. Chem, V54, P2410

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