Discovery of Antitumor Active Peptides Derived from Peroxiredoxin 5

被引：2

作者：

Liu, Juanjuan ^{[1
,2
]}

Zou, Sen ^{[1
,2
]}

Zhang, Yan ^{[3
]}

Lin, Ru ^{[1
,2
]}

Duan, Yanbo ^{[3
]}

He, Weiqing ^{[1
,2
]}

Yang, Zhaoyong ^{[1
,2
]}

机构：

[1] Chinese Acad Med Sci, Inst Med Biotechnol, 1 Tiantanxili, Beijing, Peoples R China

[2] Peking Union Med Coll, 1 Tiantanxili, Beijing, Peoples R China

[3] Shen Yang Tonglian Grp Co Ltd, Shenyang, Peoples R China

来源：

CHEMMEDCHEM | 2021年 / 16卷 / 22期

基金：

中国国家自然科学基金;

关键词：

peptides; peroxiredoxin-5; antioxidation; antitumor agents; transcriptome analysis; INTRAMOLECULAR DISULFIDE; MAMMALIAN PEROXIREDOXIN; MECHANISM; IDENTIFICATION; APOPTOSIS; PATHWAYS; GENOMES; FORMS; KEGG;

D O I：

10.1002/cmdc.202100323

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

The peroxiredoxin 5 (PRDX5) is a member of peroxiredoxins with antitumor activity. However, as a recombinant protein, PRDX5 is restricted in clinic due to high cost and keeping high dose in medication. The alternative way is to explore the antitumor active fragments of PRDX5 for potential of peptide drugs. According to the sequence, crystal structure and enzyme function of PRDX5, seven peptides were designed and named as IMB-P1 similar to 7. The peptide IMB-P1 (AFTPGCSKTHLPGFVEQAEAL) containing critical residue C47 exhibited antitumor activity similar to PRDX5 in vivo. Transcriptome analysis showed peptide IMB-P1 could make influence on expression of multiple genes involved in tumorigenesis and deterioration. Besides, an important discovery is the down-regulation of oxidation-related genes. In CT26 cells, IMB-P1 carried similar antitumor activity with increasing ROS level to intact PRDX5. The results demonstrated that peptide IMB-P1 with easier synthesis from PRDX5 may serve as a promising antitumor candidate.

引用

页码：3477 / 3483

页数：7

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