Oil Red O Staining for Lipid Content in Caenorhabditis elegans

被引：20
|
作者：
Wang, Feng-Yung [1 ]
Ching, Tsui-Ting [2 ]
机构：
[1] Natl Yang Ming Chiao Tung Univ, Inst Biochem & Mol Biol, Taipei, Taiwan
[2] Natl Yang Ming Chiao Tung Univ, Inst Biopharmaceut Sci, Taipei, Taiwan
来源：
BIO-PROTOCOL | 2021年 / 11卷 / 16期
关键词：
Oil Red O; Fat; Lipid metabolism; C; elegans; GENETICS; ADIPOSE; MODEL;
D O I：
10.21769/BioProtoc.4124
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The nematode Caenorhabditis elegans has emerged as a popular model system for studying the regulation of lipid metabolism. Therefore, it is critical to develop a method for determining fat storage in individual worms. Oil Red O (ORO) staining has been validated as an accurate assessment for major fat storage in C. elegans. Here, we describe an optimized protocol for ORO staining of C. elegans and provide detailed instructions for quantifying the intensity of ORO signal in images acquired by light microscopy.
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页数：9
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