Association of MDR-TB isolates with clinical characteristics of patients from Northern region of India

被引：9

作者：

Gupta, A. ^{[1
]}

Nagaraja, M. R. ^{[2
,6
]}

Kumari, P. ^{[4
,5
]}

Singh, G. ^{[3
]}

Raman, R. ^{[4
,5
]}

Singh, S. K. ^{[2
]}

Anupurba, S. ^{[1
]}

机构：

[1] Banaras Hindu Univ, Dept Microbiol, Varanasi 221005, Uttar Pradesh, India

[2] Banaras Hindu Univ, Dept Endocrinol & Metab, Varanasi 221005, Uttar Pradesh, India

[3] Banaras Hindu Univ, Div Biostat, Varanasi 221005, Uttar Pradesh, India

[4] Banaras Hindu Univ, Inst Med Sci, Dept Zool, Varanasi 221005, Uttar Pradesh, India

[5] Banaras Hindu Univ, Ctr Genet Disorder, Varanasi 221005, Uttar Pradesh, India

[6] Chikka Muniyappa Reddy Inst Management Studies, Dept Biochem, Bangalore, Karnataka, India

来源：

INDIAN JOURNAL OF MEDICAL MICROBIOLOGY | 2014年 / 32卷 / 03期

关键词：

Deoxyribonucleic Acid sequencing; Human Immunodeficiency Virus; Multidrug-resistant-tuberculosis; Polymerase Chain Reaction-single-strand conformation polymorphism; tuberculosis; RESISTANT MYCOBACTERIUM-TUBERCULOSIS; POLYMERASE-CHAIN-REACTION; RIFAMPIN RESISTANCE; DRUG SENSITIVITY; RPOB; MUTATIONS; STRAINS; PCR; IDENTIFICATION; OUTCOMES;

D O I：

10.4103/0255-0857.136561

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Purpose: We sought to determine the characteristics and relative frequency of transmission of MDR-TB in North India and their association with the clinical and epidemiological characteristics of TB-patients. Materials and Methods: To achieve the objectives PCR-SSCP, MAS-PCR and direct DNA sequencing were used against 101 Mycobacterium tuberculosis isolates. Results: Multidrug-resistant-TB isolates were found to be significantly higher (P = 0.000) in previously treated patients in comparison to newly diagnosed patients. Further, significant differences (P = 0.003) were observed between different age groups (Mean +/- SD, 28.6 +/- 11.77) of the TB patients and multidrug resistance. Most frequent mutations were observed at codons 531 and 315 of rpoB and katG genes, respectively, in MDR-TB isolates. Conclusion: Routine surveillance of resistance to anti-TB drugs will improve timely recognition of MDR-TB cases and help prevent further transmission in Northern India.

引用

页码：270 / 276

页数：7

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