Detection of Shiga Toxins by Lateral Flow Assay

被引：25

作者：

Ching, Kathryn H. ^{[1
]}

He, Xiaohua ^{[2
]}

Stanker, Larry H. ^{[2
]}

Lin, Alice V. ^{[1
]}

McGarvey, Jeffery A. ^{[2
]}

Hnasko, Robert ^{[1
]}

机构：

[1] ARS, Produce Safety & Microbiol Res Unit, USDA, Albany, CA 94710 USA

[2] ARS, Foodborne Toxin Detect & Prevent Res Unit, USDA, Albany, CA 94710 USA

来源：

TOXINS | 2015年 / 7卷 / 04期

关键词：

ESCHERICHIA-COLI O157-H7; UNITED-STATES; INFECTIONS; VARIANT; IDENTIFICATION; EPIDEMIOLOGY; PREVALENCE; DISEASE;

D O I：

10.3390/toxins7041163

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Shiga toxin-producing Escherichia coli (STEC) produce shiga toxins (Stxs) that can cause human disease and death. The contamination of food products with STEC represents a food safety problem that necessitates rapid and effective detection strategies to mitigate risk. In this manuscript, we report the development of a colorimetric lateral flow assay (LFA) for the rapid detection of Stxs in <10 min using a pair of monoclonal antibodies that bind epitopes common to Stx1 and six Stx2 variants. This LFA provides a rapid and sensitive test for the detection of Stxs directly from STEC culture supernatants or at risk food samples with a 0.1 ng/mL limit of detection (LOD) for Stx2a. This Stx LFA is applicable for use in the rapid evaluation of Stx production from cultured E. coli strains or as a tool to augment current methods as part of food safety testing.

引用

页码：1163 / 1173

页数：11

共 32 条

[21] Escherichia coli O157 [J].

Pennington, Hugh .

LANCET, 2010, 376 (9750) :1428-1435

[22]

Piérard D, 1998, J CLIN MICROBIOL, V36, P3317

[23]

Pihkala Nora., 2012, RISK PROFILE PATHOGE

[24] Lateral flow (immuno) assay: its strengths, weaknesses, opportunities and threats. A literature survey [J].

Posthuma-Trumpie, Geertruida A. ;

Korf, Jakob ;

van Amerongen, Aart .

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2009, 393 (02) :569-582

[25] Epidemiology of Escherichia coli O157:H7 outbreaks, United States, 1982-2002 [J].

Rangel, JM ;

Sparling, PH ;

Crowe, C ;

Griffin, PM ;

Swerdlow, DL .

EMERGING INFECTIOUS DISEASES, 2005, 11 (04) :603-609

[26]

Scallan E, 2011, EMERG INFECT DIS, V17, P16, DOI [10.3201/eid1701.P21101, 10.3201/eid1701.091101p2]

[27] A new Shiga toxin 2 variant (Stx2f) from Escherichia coli isolated from pigeons [J].

Schmidt, H ;

Scheef, J ;

Morabito, S ;

Caprioli, A ;

Wieler, LH ;

Karch, H .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2000, 66 (03) :1205-1208

[28] Infections associated with eating seed sprouts: An international concern [J].

Taormina, PJ ;

Beuchat, LR ;

Slutsker, L .

EMERGING INFECTIOUS DISEASES, 1999, 5 (05) :626-634

[29] Rapid detection of Bacillus anthracis spores using a super-paramagnetic lateral-flow immunological detection system [J].

Wang, Dian-Bing ;

Tian, Bo ;

Zhang, Zhi-Ping ;

Deng, Jiao-Yu ;

Cui, Zong-Qiang ;

Yang, Rui-Fu ;

Wang, Xu-Ying ;

Wei, Hong-Ping ;

Zhang, Xian-En .

BIOSENSORS & BIOELECTRONICS, 2013, 42 :661-667

[30] CLONING AND SEQUENCING OF A SHIGA-LIKE TOXIN TYPE-II VARIANT FROM AN ESCHERICHIA-COLI STRAIN RESPONSIBLE FOR EDEMA DISEASE OF SWINE [J].

WEINSTEIN, DL ;

JACKSON, MP ;

SAMUEL, JE ;

HOLMES, RK ;

OBRIEN, AD .

JOURNAL OF BACTERIOLOGY, 1988, 170 (09) :4223-4230

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