Detection of Shiga Toxins by Lateral Flow Assay

被引：25

作者：

Ching, Kathryn H. ^{[1
]}

He, Xiaohua ^{[2
]}

Stanker, Larry H. ^{[2
]}

Lin, Alice V. ^{[1
]}

McGarvey, Jeffery A. ^{[2
]}

Hnasko, Robert ^{[1
]}

机构：

[1] ARS, Produce Safety & Microbiol Res Unit, USDA, Albany, CA 94710 USA

[2] ARS, Foodborne Toxin Detect & Prevent Res Unit, USDA, Albany, CA 94710 USA

来源：

TOXINS | 2015年 / 7卷 / 04期

关键词：

ESCHERICHIA-COLI O157-H7; UNITED-STATES; INFECTIONS; VARIANT; IDENTIFICATION; EPIDEMIOLOGY; PREVALENCE; DISEASE;

D O I：

10.3390/toxins7041163

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Shiga toxin-producing Escherichia coli (STEC) produce shiga toxins (Stxs) that can cause human disease and death. The contamination of food products with STEC represents a food safety problem that necessitates rapid and effective detection strategies to mitigate risk. In this manuscript, we report the development of a colorimetric lateral flow assay (LFA) for the rapid detection of Stxs in <10 min using a pair of monoclonal antibodies that bind epitopes common to Stx1 and six Stx2 variants. This LFA provides a rapid and sensitive test for the detection of Stxs directly from STEC culture supernatants or at risk food samples with a 0.1 ng/mL limit of detection (LOD) for Stx2a. This Stx LFA is applicable for use in the rapid evaluation of Stx production from cultured E. coli strains or as a tool to augment current methods as part of food safety testing.

引用

页码：1163 / 1173

页数：11

共 32 条

[1] Fresh fruit and vegetables as vehicles for the transmission of human pathogens [J].

Berger, Cedric N. ;

Sodha, Samir V. ;

Shaw, Robert K. ;

Griffin, Patricia M. ;

Pink, David ;

Hand, Paul ;

Frankel, Gad .

ENVIRONMENTAL MICROBIOLOGY, 2010, 12 (09) :2385-2397

[2]

Besser RE, 1999, ANNU REV MED, V50, P355, DOI 10.1146/annurev.med.50.1.355

[3] Rapid laboratory identification and characterization of verocytotoxigenic (Shiga toxin producing) Escherichia coli (VTEC/STEC) [J].

Bettelheim, KA ;

Beutin, L .

JOURNAL OF APPLIED MICROBIOLOGY, 2003, 95 (02) :205-217

[4] Associations between virulence factors of Shiga toxin-producing Escherichia coli and disease in humans [J].

Boerlin, P ;

McEwen, SA ;

Boerlin-Petzold, F ;

Wilson, JB ;

Johnson, RP ;

Gyles, CL .

JOURNAL OF CLINICAL MICROBIOLOGY, 1999, 37 (03) :497-503

[5] Non-O157 shiga toxin-producing Escherichia coli infections in the United States, 1983-2002 [J].

Brooks, JT ;

Sowers, EG ;

Wells, JG ;

Greene, KD ;

Griffin, PM ;

Hoekstra, RM ;

Strockbine, NA .

JOURNAL OF INFECTIOUS DISEASES, 2005, 192 (08) :1422-1429

[6] Rapid and selective detection of botulinum neurotoxin serotype-A and -B with a single immunochromatographic test strip [J].

Ching, Kathryn H. ;

Lin, Alice ;

McGarvey, Jeffery A. ;

Stanker, Larry H. ;

Hnasko, Robert .

JOURNAL OF IMMUNOLOGICAL METHODS, 2012, 380 (1-2) :23-29

[7] Incidence and Tracking of Escherichia coli O157:H7 in a Major Produce Production Region in California [J].

Cooley, Michael ;

Carychao, Diana ;

Crawford-Miksza, Leta ;

Jay, Michele T. ;

Myers, Carol ;

Rose, Christopher ;

Keys, Christine ;

Farrar, Jeff ;

Mandrell, Robert E. .

PLOS ONE, 2007, 2 (11)

[8]

Eblen D., 2007, PUBLIC HLTH IMPORTAN

[9] SITE OF ACTION OF A VERO TOXIN (VT2) FROM ESCHERICHIA-COLI O157-H7 AND OF SHIGA TOXIN ON EUKARYOTIC RIBOSOMES - RNA N-GLYCOSIDASE ACTIVITY OF THE TOXINS [J].

ENDO, Y ;

TSURUGI, K ;

YUTSUDO, T ;

TAKEDA, Y ;

OGASAWARA, T ;

IGARASHI, K .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1988, 171 (1-2) :45-50

[10] Evaluation of lateral flow assays for the detection of botulinum neurotoxin type A and their application in laboratory diagnosis of botulism [J].

Gessler, Frank ;

Pagel-Wieder, Sibylle ;

Avondet, Marc-Andre ;

Boehnel, Helge .

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2007, 57 (03) :243-249

← 1 2 3 4 →