Selection of potato clones for multiple resistances to early blight, PVY and PVX

被引:7
作者
Neder, Diogo Goncalves [1 ]
Brasil Pereira Pinto, Cesar Augusto [2 ]
Melo, Dheyne Silva [3 ]
Lepre, Andre Luiz [4 ]
Peixouto, Leandro dos Santos [5 ]
机构
[1] Univ Fed Rural Pernambuco DEPA UFRPE, Dept Agron, BR-52171900 Recife, PE, Brazil
[2] Univ Fed Lavras UFLA, Lavras, MG, Brazil
[3] CTC, Piracicaba, SP, Brazil
[4] Geneze Sementes, Paracatu, MG, Brazil
[5] Inst Fed Educ Ciencia & Tecnol Baiano IFBAIANO, Guanambi, BA, Brazil
来源
CIENCIA RURAL | 2010年 / 40卷 / 08期
关键词
evaluate agronomic; Marker assisted selection; genetic resistance; Potato virus X; Potato virus Y; Alternaria solani; EXTREME RESISTANCE; CHROMOSOME-XII; GENE;
D O I
10.1590/S0103-84782010000800005
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
The purpose of this study was to select potato clones with high agronomic performance and resistant to early blight, Potato Virus Y (PVY) and Potato Virus X (PVX). Crossings were done among progenitors carrying the Ry(adg) and Rx1 alleles for resistance to PVY and PVX and the cultivar 'Chiquita', which presents high levels of resistance to early blight (Alternaria solani). In the rainy season of 2004, 57 clonal families were evaluated in the field and 331 clones were selected based on tuber appearance. These clones were field evaluated in two trials in the rainy season of 2005 and 180 clones were selected for Ry(adg) allele with the SCAR marker designed RYSC3. Another agronomic evaluation was done in the winter season of 2006 and early blight was evaluated in the rainy seasons of 2007 and 2008. Simultaneously a CAPS marker was used to select for the presence of Rx1 allele. Combining the results from these experiments we were able to select 20 clones presenting high agronomic performance, resistance to early blight and carrying the Ry(adg) allele. The use of CAPS marker has practical difficulties due to production of poor amplification products to be digested with the Dde1 enzyme and should be changed for another marker which shows more efficiency.
引用
收藏
页码:1702 / 1708
页数:7
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