RETRACTED: The In Vitro Antimicrobial and Antibiofilm Activities of Lysozyme against Gram-Positive Bacteria (Retracted Article)

被引：2

作者：

Liu, Fang ^{[1
]}

Wang, Xing ^{[2
]}

Huang, Lijie ^{[2
]}

Wang, Xinling ^{[2
]}

Kong, Lili ^{[2
]}

Duan, Jinyou ^{[2
]}

Zhang, Xiaoli ^{[2
]}

Mu, Haibo ^{[2
]}

He, Jianguo ^{[3
]}

机构：

[1] Chongqing Red Cross Hosp, Peoples Hosp Jiangbei Dist, Dept Clin Lab, Chongqing 400020, Peoples R China

[2] Northwest A&F Univ, Coll Chem & Pharm, Shaanxi Key Lab Nat Prod & Chem Biol, Yangling 712100, Shaanxi, Peoples R China

[3] Chongqing Red Cross Hosp, Peoples Hosp Jiangbei Dist, Dept Neurosurg, Chongqing 400020, Peoples R China

来源：

COMPUTATIONAL AND MATHEMATICAL METHODS IN MEDICINE | 2022年 / 2022卷

基金：

中国国家自然科学基金;

关键词：

BIOFILM FORMATION; RESISTANCE; INFECTIONS; VANCOMYCIN; VIRULENCE;

D O I：

10.1155/2022/4559982

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Objective. To analyze the in vitro antibacterial and antibiofilm activities of lysozyme (LYS) and its combination with various drugs against Gram-positive bacteria (GPB, n=9), thus to provide an exploration direction for drug development. Methods. The minimum inhibitory concentrations (MICs) of linezolid (LZD), amikacin (AMK), ceftriaxone/sulbactam (CRO/SBT), cefotaxime/sulbactam (CTX/SBT), piperacillin/sulbactam (PIP/SBT), doxycycline (DOX), levofloxacin (LVX), amoxicillin/clavulanate potassium (7 : 1, AK71), imipenem (IPM), azithromycin (AZM), and their combinations with LYS were determined with tuber twice dilution. The antimicrobial and antibiofilm activities of LYS, AZM, LVX, and their combinations with others were evaluated through MTT and crystal violet assay. Results. High-dose LYS (30 mu g/mL) combined with PIP/SBT and AK71, respectively, showed synergistic antibacterial activities against methicillin-resistant Staphylococcus aureus (MRSA), while it showed no synergistic activities when combined with other drugs. LYS and AZM inhibited the biofilm formation of one MRSA strain, but they and LVX had no similar activities against methicillin-resistant Staphylococcus epidermidis (MRSE) or vancomycin-resistant Enterococcus faecium (VREF). Particularly, LYS increased the permeability of biofilms of MRSA 33 and exhibited antibiofilm activities against MRSA 31 (inhibition rate=38.1%) and MRSE 61 (inhibition rate=46.6%). The combinations of PIP/SBT+LYS, AMK+LYS, and LZD+LYS showed stronger antibiofilm activities against MRSA 62, MRSE 62, MRSE 63, and VREF 11. Conclusion. The antimicrobial and antibiofilm activities of LYS against MRSA were better than AZM, while that of LYS against MRSE and VREF, respectively, was similar with AZM and LVX.

引用

页数：10

共 40 条

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