Fourier Transform Infrared Spectroscopy and Photoacoustic Spectroscopy for Saliva Analysis

被引：23

作者：

Mikkonen, Jopi J. W. ^{[1
,2
,3
]}

Raittila, Jussi ^{[4
]}

Rieppo, Lassi ^{[5
,6
,7
]}

Lappalainen, Reijo ^{[1
,2
]}

Kullaa, Arja M. ^{[3
,7
,8
,9
]}

Myllymaa, Sami ^{[1
,2
,3
]}

机构：

[1] Univ Eastern Finland, SIB Labs, Yliopistonranta 1, FI-70211 Kuopio, Finland

[2] Univ Eastern Finland, Fac Sci & Forestry, Dept Appl Phys, Kuopio, Finland

[3] Univ Eastern Finland, Inst Dent, Kuopio, Finland

[4] Gasera Ltd, Turku, Finland

[5] Univ Oulu, Fac Med, Res Unit Med Imaging Phys & Technol, Oulu, Finland

[6] Univ Oulu, Med Res Ctr, Oulu, Finland

[7] Oulu Univ Hosp, Oulu, Finland

[8] Univ Oulu, Fac Med, Res Grp Oral Hlth Sci, Oulu, Finland

[9] Kuopio Univ Hosp, Educ Dent Clin, Kuopio, Finland

来源：

APPLIED SPECTROSCOPY | 2016年 / 70卷 / 09期

关键词：

Infrared; IR; spectroscopy; photoacoustic; PA; fourier transform infrared spectroscopy; FT-IR; oral cancer; oral diseases; saliva analysis; HYDROGEN-PEROXIDE TOXICITY; MOLECULAR SIGNATURES; FTIR SPECTROSCOPY; BIOLOGICAL-FLUIDS; IR SPECTROSCOPY; OVARIAN-CANCER; SERUM SAMPLES; REAGENT-FREE; THIOCYANATE; SPECTRA;

D O I：

10.1177/0003702816654149

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

Saliva provides a valuable tool for assessing oral and systemic diseases, but concentrations of salivary components are very small, calling the need for precise analysis methods. In this work, Fourier transform infrared (FT-IR) spectroscopy using transmission and photoacoustic (PA) modes were compared for quantitative analysis of saliva. The performance of these techniques was compared with a calibration series. The linearity of spectrum output was verified by using albumin-thiocyanate (SCN-) solution at different SCN- concentrations. Saliva samples used as a comparison were obtained from healthy subjects. Saliva droplets of 15 mu L were applied on the silicon sample substrate, 6 drops for each specimen, and dried at 37? overnight. The measurements were carried out using an FT-IR spectrometer in conjunction with an accessory unit for PA measurements. The findings with both transmission and PA modes mirror each other. The major bands presented were 1500-1750cm(-1) for proteins and 1050-1200cm(-1) for carbohydrates. In addition, the distinct spectral band at 2050cm(-1) derives from SCN- anions, which is converted by salivary peroxidases to hypothiocyanate (OSCN-). The correlation between the spectroscopic data with SCN- concentration (r>0.990 for transmission and r=0.967 for PA mode) was found to be significant (P<0.01), thus promising to be utilized in future applications.

引用

页码：1502 / 1510

页数：9