Role of phosphorylation clusters in the biology of the coronavirus infectious bronchitis virus nucleocapsid protein

被引：37
作者：
Spencer, Kelly-Anne [1 ]
Dee, Michael [2 ]
Britton, Paul [2 ]
Hiscox, Julian A. [1 ,3 ]
机构：
[1] Univ Leeds, Fac Biol Sci, Inst Mol & Cellular Biol, Leeds LS2 9JT, W Yorkshire, England
[2] Inst Anim Hlth, Div Microbiol, Newbury RG20 7NN, Berks, England
[3] Univ Leeds, Astbury Ctr Struct Mol Biol, Leeds LS2 9JT, W Yorkshire, England
来源：
VIROLOGY | 2008年 / 370卷 / 02期
基金：
英国生物技术与生命科学研究理事会;
关键词：
coronavirus; RNA binding; nucleocapsid protein; IBV; surface plasmon resonance; phosphorylation;
D O I：
10.1016/j.virol.2007.08.016
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The coronavirus infectious bronchitis virus (IBV) nucleocapsid (N) protein is an RNA binding protein which is phosphorylated at two conserved clusters. Kinetic analysis of RNA binding indicated that the C-terminal phosphorylation cluster was involved in the recognition of viral RNA from non-viral RNA. The IBV N protein has been found to be essential for the successful recovery of IBV using reverse genetics systems. Rescue experiments indicated that phosphorylated N protein recovered infectious IBV more efficiently when compared to modified N proteins either partially or non-phosphorylated. Our data indicate that the phosphorylated form of the IBV N protein plays a role in virus biology. (c) 2007 Elsevier Inc. All rights reserved.
引用
收藏
页码：373 / 381
页数：9
相关论文
共 50 条
  • [21] Identification of phosphorylation sites in the nucleocapsid protein (N protein) of SARS-coronavirus
    Lin, Liang
    Shao, Jianmin
    Sun, Maomao
    Liu, Jinxiu
    Xu, Gongjin
    Zhang, Xumin
    Xu, Ningzhi
    Wang, Rong
    Liu, Siqi
    INTERNATIONAL JOURNAL OF MASS SPECTROMETRY, 2007, 268 (2-3) : 296 - 303
  • [22] Generation of a recombinant avian coronavirus infectious bronchitis virus using transient dominant selection
    Britton, P
    Evans, S
    Dove, B
    Davies, M
    Casais, R
    Cavanagh, D
    JOURNAL OF VIROLOGICAL METHODS, 2005, 123 (02) : 203 - 211
  • [23] The nucleocapsid protein of SARS-associated coronavirus inhibits B23 phosphorylation
    Zeng, Yingchun
    Ye, Linbai
    Zhu, Shengli
    Zheng, Hong
    Zhao, Peng
    Cai, Weijia
    Su, Liya
    She, Yinglong
    Wu, Zhenghui
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2008, 369 (02) : 287 - 291
  • [24] Replicase 1a gene plays a critical role in pathogenesis of avian coronavirus infectious bronchitis virus
    Zhao, Jing
    Zhang, Keran
    Cheng, Jinlong
    Jia, Wenfeng
    Zhao, Ye
    Zhang, Guozhong
    VIROLOGY, 2020, 550 : 1 - 7
  • [25] Anti-Infectious Bronchitis Virus (IBV) Activity of 1,8-cineole: Effect on Nucleocapsid (N) Protein
    Yang, Zhiwei
    Wu, Nan
    Fu, Yujie
    Yang, Gang
    Wang, Wei
    Zu, Yuangang
    Efferth, Thomas
    JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS, 2010, 28 (03) : 323 - 330
  • [26] Evolution of infectious bronchitis virus in Taiwan: Positively selected sites in the nucleocapsid protein and their effects on RNA-binding activity
    Kuo, Shu-Ming
    Kao, Hsiao-Wei
    Hou, Ming-Hon
    Wang, Ching-Ho
    Lin, Siou-Hong
    Su, Hong-Lin
    VETERINARY MICROBIOLOGY, 2013, 162 (2-4) : 408 - 418
  • [27] Enhancement of the immunogenicity of an infectious bronchitis virus DNA vaccine by a bicistronic plasmid encoding nucleocapsid protein and interleukin-2
    Tang, Mengjun
    Wang, Hongning
    Zhou, Sheng
    Tian, Guobao
    JOURNAL OF VIROLOGICAL METHODS, 2008, 149 (01) : 42 - 48
  • [28] Temperature Sensitivity: A Potential Method for the Generation of Vaccines against the Avian Coronavirus Infectious Bronchitis Virus
    Keep, Sarah
    Stevenson-Leggett, Phoebe
    Steyn, Angela
    Oade, Michael S.
    Webb, Isobel
    Stuart, Jamie
    Vervelde, Lonneke
    Britton, Paul
    Maier, Helena J.
    Bickerton, Erica
    VIRUSES-BASEL, 2020, 12 (07):
  • [29] Chicken interferon-induced transmembrane protein 1 promotes replication of coronavirus infectious bronchitis virus in a cell-specific manner
    Li, Hao
    Ni, Ruiqi
    Wang, Kailu
    Tian, Yiming
    Gong, Huilin
    Yan, Wenjun
    Tang, Yizhi
    Lei, Changwei
    Wang, Hongning
    Yang, Xin
    VETERINARY MICROBIOLOGY, 2022, 275
  • [30] Role of VP30 Phosphorylation in Ebola Virus Nucleocapsid Assembly and Transport
    Takamatsu, Yuki
    Yoshikawa, Tomoki
    Kurosu, Takeshi
    Fukushi, Shuetsu
    Nagata, Noriyo
    Shimojima, Masayuki
    Ebihara, Hideki
    Saijo, Masayuki
    Noda, Takeshi
    JOURNAL OF VIROLOGY, 2022, 96 (17)
12345