A bHLH transcription factor from Chenopodium glaucum confers drought tolerance to transgenic maize by positive regulation of morphological and physiological performances and stress-responsive genes' expressions

被引：10

作者：

Zhao, Haiju ^{[1
]}

Wang, Changhai ^{[2
]}

Lan, Haiyan ^{[1
]}

机构：

[1] Xinjiang Univ, Coll Life Sci & Technol, Xinjiang Key Lab Biol Resources & Genet Engn, Urumqi 830017, Peoples R China

[2] Join Hope Seeds Ind Co Ltd, Changji 831199, Peoples R China

来源：

MOLECULAR BREEDING | 2021年 / 41卷 / 12期

基金：

中国国家自然科学基金;

关键词：

bHLH transcription factor; Drought-related genes; Drought tolerance; RNAi; Transgenic maize; SELECTABLE MARKER GENE; REAL-TIME PCR; COPY NUMBER; FUNCTIONAL-ANALYSIS; RNA INTERFERENCE; SALT TOLERANCE; ARABIDOPSIS; OVEREXPRESSION; YIELD; RESISTANCE;

D O I：

10.1007/s11032-021-01267-4

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

The basic helix-loop-helix (bHLH) transcription factor has been shown to play an important role in various physiological processes. However, its functions and mechanisms in drought tolerance still remain poorly understood. Here, we reported a bHLH transcription factor - CgbHLH001 - from Chenopodium glaucum, which was able to confer drought tolerance in maize. CgbHLH001-overexpressed maize lines exhibited drought-tolerant phenotype and improved ear traits by accumulating the contents of soluble sugar and proline and elevating the activities of antioxidant enzymes (SOD, POD, and CAT) under drought stress, accompanying with the upregulation of some stress-related genes, which may balance the redox and osmotic homeostasis compared with the non-transgenic and CgbHLH001-RNAi plants. These findings suggest that CgbHLH001 can confer drought tolerance and has the potential for utilization in improving drought resistance in maize breeding.

引用

页数：18

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