Agrobacterium-mediated genetic transformation of grapevine (Vitis vinifera L.) with a novel stilbene synthase gene from Chinese wild Vitis pseudoreticulata

被引：63

作者：

Fan, Chaohong ^{[1
,2
,3
]}

Pu, Ni ^{[1
,2
,3
]}

Wang, Xiping ^{[1
,2
,3
]}

Wang, Yuejin ^{[1
,2
,3
]}

Fang, Li ^{[1
,2
,3
]}

Xu, Weirong ^{[1
,2
,3
]}

Zhang, Jianxia ^{[1
,2
,3
]}

机构：

[1] NW A & F Univ, Coll Hort, Yangling 712100, Peoples R China

[2] Key Lab Mol Biol Agr, Yangling, Peoples R China

[3] Key Lab Hort Plant Germplasm Resources & Genet Im, Minist Agr China, Yangling, Peoples R China

来源：

PLANT CELL TISSUE AND ORGAN CULTURE | 2008年 / 92卷 / 02期

基金：

中国国家自然科学基金;

关键词：

genetic transformation; grapevine; resveratrol; somatic embryogenesis; stilbene synthase gene; Vitis;

D O I：

10.1007/s11240-007-9324-2

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A novel stilbene synthase gene (STS), cloned from Chinese wild Vitis pseudoreticulata (W. T. Wang) and responsible for synthesis of the phytoalexin resveratrol in grapevine, was successfully transferred into V. vinifera L. cv. Thompson Seedless via Agrobacterium tumefaciens-mediated transformation. Using transformation procedures developed in the present study, 72% GFP-positive germinated embryos were produced with about 38% of transformed embryos regenerated into normal plantlets. Integration of the STS gene into the transgenic plants was verified by PCR and Southern blot analysis. Expression of the STS gene was detected by high performance liquid chromatography (HPLC), which showed that the resveratrol concentration in the transgenic plants was 5.5 times higher than that in non-transformed control plants.

引用

页码：197 / 206

页数：10

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