Palmitoylation of POTE family proteins for plasma membrane targeting

被引：6

作者：

Das, Sudipto ^{[1
]}

Ise, Tomoko ^{[1
]}

Nagata, Satoshi ^{[1
]}

Maeda, Hiroshi ^{[1
]}

Bera, Tapan K. ^{[1
]}

Pastan, Ira ^{[1
]}

机构：

[1] NCI, Mol Biol Lab, Canc Res Ctr, NIH, Bethesda, MD 20892 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2007年 / 363卷 / 03期

关键词：

prostate; evolution; protein modification; plasma membrane; palmitoylation; S-acylation; subcellular localization; spectrin; ankyrin;

D O I：

10.1016/j.bbrc.2007.09.045

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The POTE gene family is composed of 13 paralogs and likely evolved by duplications and remodeling of the human genome. One common property of POTE proteins is their localization on the inner aspect of the plasma membrane. To determine the structural elements required for membrane localization, we expressed mutants of different POTEs in 293T cells as EGFP fusion proteins. We also tested their palmitoylation by a biotin-switch assay. Our data indicate that the membrane localizations of different POTEs are mediated by similar 3-4 short cysteine rich repeats (CRRs) near the amino-terminuses and that palmitoylation on paired cysteine residues in each CRR motif is responsible for the localization. Multiple palmitoylation in the small CRRs can result in the strong association of whole POTEs with plasma membrane. Published by Elsevier Inc.

引用

页码：751 / 756

页数：6

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