Voltage gating is a fundamental feature of porin and toxin β-barrel membrane channels

被引：77

作者：

Bainbridge, G ^{[1
]}

Gokce, I ^{[1
]}

Lakey, JH ^{[1
]}

机构：

[1] Univ Newcastle Upon Tyne, Sch Med, Dept Biochem & Genet, Newcastle Upon Tyne NE2 4HH, Tyne & Wear, England

来源：

FEBS LETTERS | 1998年 / 431卷 / 03期

基金：

英国生物技术与生命科学研究理事会;

关键词：

beta-barrel pore; voltage gating;

D O I：

10.1016/S0014-5793(98)00761-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Beta-barrel pores are found in outer membrane porins of Gram-negative bacteria, bacterial toxins and mitochondrial channels. Apart from the beta-barrel the three groups show no close sequence or structural homology but these pores exhibit symmetrical voltage gating when reconstituted into planar lipid bilayers. The structures of several of these are known and many site-directed mutants have been examined. As a result it seems evident that the gating is a common characteristic of these unrelated large pores and is not generated by specialised structures in the pore lumen. (C) 1998 Federation of European Biochemical Societies.

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收藏

页码：305 / 308

页数：4

相关论文

共 55 条

[41] Structural and functional characterization of OmpF porin mutants selected for larger pore size .2. Functional characterization [J].

Saint, N ;

Lou, KL ;

Widmer, C ;

Luckey, M ;

Schirmer, T ;

Rosenbusch, JP .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (34) :20676-20680

[42] Replacement of the sole histidinyl residue in OmpF porin from E-coli by threonine (H21T) does not affect channel structure and function [J].

Saint, N ;

Prilipov, A ;

Hardmeyer, A ;

Lou, KL ;

Schirmer, T ;

Rosenbusch, JP .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1996, 223 (01) :118-122

[43] E-coli PhoE porin has an opposite voltage-dependence to the homologous OmpF [J].

Samartzidou, H ;

Delcour, AH .

EMBO JOURNAL, 1998, 17 (01) :93-100

[44] MATRIX PROTEIN FROM ESCHERICHIA-COLI OUTER MEMBRANES FORMS VOLTAGE-CONTROLLED CHANNELS IN LIPID BILAYERS [J].

SCHINDLER, H ;

ROSENBUSCH, JP .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1978, 75 (08) :3751-3755

[45] STRUCTURAL BASIS FOR SUGAR TRANSLOCATION THROUGH MALTOPORIN CHANNELS AT 3.1-ANGSTROM RESOLUTION [J].

SCHIRMER, T ;

KELLER, TA ;

WANG, YF ;

ROSENBUSCH, JP .

SCIENCE, 1995, 267 (5197) :512-514

[46] PERTURBATION-RELAXATION MOLECULAR-DYNAMICS SIMULATIONS AS A TOOL TO EXPLORE CONFORMATIONAL SPACE - REVERSIBLE RESPONSE OF THE L3 LOOP IN PORIN TOWARDS CHARGE SCREENING EFFECTS [J].

SOARES, CM ;

BJORKSTEN, J ;

TAPIA, O .

MOLECULAR SIMULATION, 1995, 15 (01) :35-46

[47] Structure of staphylococcal alpha-hemolysin, a heptameric transmembrane pore [J].

Song, LZ ;

Hobaugh, MR ;

Shustak, C ;

Cheley, S ;

Bayley, H ;

Gouaux, JE .

SCIENCE, 1996, 274 (5294) :1859-1866

[48]

TRIAS JQ, 1993, J BIOL CHEM, V268, P6234

[49]

VACHON V, 1986, BIOCHIM BIOPHYS ACTA, V861, P74, DOI 10.1016/0005-2736(86)90405-0

[50] Voltage sensing in the PhoE and OmpF outer membrane porins of Escherichia coli: Role of charged residues [J].

VanGelder, P ;

Saint, N ;

Phale, P ;

Eppens, EF ;

Prilipov, A ;

vanBoxtel, R ;

Rosenbusch, JP ;

Tommassen, J .

JOURNAL OF MOLECULAR BIOLOGY, 1997, 269 (04) :468-472

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