Fast and Specific Screening of EPA/DHA-Enriched Phospholipids in Fish Oil Extracted from Different Species by HILIC-MS

被引：15

作者：

Yu, Xina ^{[1
,2
]}

Wang, Qingcheng ^{[3
]}

Lu, Weibo ^{[1
]}

Zhang, Min ^{[1
]}

Chen, Kang ^{[1
]}

Xue, Jing ^{[1
]}

Zhao, Qiaoling ^{[4
]}

Wang, Pingya ^{[4
]}

Luo, Pei ^{[2
]}

Shen, Qing ^{[1
,2
]}

机构：

[1] Zhejiang Gongshang Univ, Collaborat Innovat Ctr Seafood Deep Proc, Inst Seafood, Zhejiang Prov Joint Key Lab Aquat Prod Proc, Hangzhou 310012, Peoples R China

[2] Macau Univ Sci & Technol, Fac Pharm, State Key Labs Qual Res Chinese Med, Macau 999078, Peoples R China

[3] Hangzhou Yuhang Hosp Tradit Chinese Med, Dept Cardiol, Yuhang 311106, Zhejiang, Peoples R China

[4] Zhoushan Inst Food & Drug Control, Zhoushan 316000, Peoples R China

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2021年 / 69卷 / 28期

关键词：

EPA/DHA-enriched phospholipids; lipidomics; fish oil; precursor ion scan; mass spectrometry; TANDEM MASS-SPECTROMETRY; TIME-OF-FLIGHT; FATTY-ACIDS; PLASMA; QUANTITATION; LIPIDOMICS; HEALTH;

D O I：

10.1021/acs.jafc.1c01709

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Eicosapentaenoic acid- and docosahexaenoic acid-enriched phospholipids (PLEPA/DHA) have versatile health-beneficial functions and can be well absorbed in the intestine. Herein, a precursor ion scan-driven hydrophilic interaction chromatography mass spectrometry (PreIS-HILIC-MS) method with the fatty acyl moieties of m/z 301.6 and 327.6 locked was established to specifically and selectively screen PLEPA/DHA in different fish oil samples, including saury, grass carp, hairtail, and yellow croaker. Taking saury oil as an example, a total of 24 PLEPA/DHA were successfully identified and quantified, including 20 PCEPA/DHA and 4 PEEPA/DHA. Finally, this method was validated in terms of sensitivity (limit of detection <= 4.15 pg.mL(-1)), linearity (>= 0.9979), precision (RSDintraday <= 4.65%), and recovery (>= 78.6%). The performance of the PreIS-HILIC-MS method was also compared with that of the traditional full-scan mode, and the former demonstrated its unique superiority in targeted screening of PLEPA/DHA in fish oils.

引用

页码：7997 / 8007

页数：11

共 46 条

[1] Absorption rate of krill oil and fish oil in blood and brain of rats [J].

Ahn, So Hyun ;

Lim, Su Jin ;

Ryu, Young Moo ;

Park, Hye-Ryung ;

Suh, Hyung Joo ;

Han, Sung Hee .

LIPIDS IN HEALTH AND DISEASE, 2018, 17

[2] Lipidomics of intact mitochondria by MALDI-TOF/MS [J].

Angelini, Roberto ;

Vitale, Rita ;

Patil, Vinay A. ;

Cocco, Tiziana ;

Ludwig, Bernd ;

Greenberg, Miriam L. ;

Corcelli, Angela .

JOURNAL OF LIPID RESEARCH, 2012, 53 (07) :1417-1425

[3] PLASMA LIPID AND LIPOPROTEIN PATTERN IN GREENLANDIC WEST-COAST ESKIMOS [J].

BANG, HO ;

DYERBERG, J ;

NIELSON, AB .

LANCET, 1971, 1 (7710) :1143-&

[4] Comparison of the Distribution of Unsaturated Fatty Acids at the Sn-2 Position of Phospholipids and Triacylglycerols in Marine Fishes and Mammals [J].

Beppu, Fumiaki ;

Yasuda, Keiko ;

Okada, Ayako ;

Hirosaki, Yoshitsugu ;

Okazaki, Masako ;

Gotoh, Naohiro .

JOURNAL OF OLEO SCIENCE, 2017, 66 (11) :1217-1227

[5]

BLIGH EG, 1959, CAN J BIOCHEM PHYS, V37, P911

[6] Functions of plasmalogen lipids in health and disease [J].

Braverman, Nancy E. ;

Moser, Ann B. .

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE, 2012, 1822 (09) :1442-1452

[7] Functions and biosynthesis of plasmalogens in health and disease [J].

Brites, P ;

Waterham, HR ;

Wanders, RJA .

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS, 2004, 1636 (2-3) :219-231

[8] Profiling and Relative Quantitation of Phosphoinositides by Multiple Precursor Ion Scanning Based on Phosphate Methylation and Isotopic Labeling [J].

Cai, Tanxi ;

Shu, Qingbo ;

Hou, JunJie ;

Liu, Peibin ;

Niu, Lili ;

Guo, Xiaojing ;

Liu, Charles C. ;

Yang, Fuquan .

ANALYTICAL CHEMISTRY, 2015, 87 (01) :513-521

[9] Using precursor ion scan of 184 with liquid chromatography-electrospray ionization-tandem mass spectrometry for concentration normalization in cellular lipidomic studies [J].

Chao, Hsi-Chun ;

Chen, Guan-Yuan ;

Hsu, Lih-Ching ;

Liao, Hsiao-Wei ;

Yang, Sin-Yu ;

Wang, San-Yuan ;

Li, Yu-Liang ;

Tang, Sung-Chun ;

Tseng, Yufeng Jane ;

Kuo, Ching-Hua .

ANALYTICA CHIMICA ACTA, 2017, 971 :68-77

[10] HPLC Determination and MS Confirmation of Malachite Green, Gentian Violet, and Their Leuco Metabolite Residues in Channel Catfish Muscle [J].

Chen, Guoying ;

Miao, Shui .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2010, 58 (12) :7109-7114

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