Effect of voluntary exercise on H2O2 release by subsarcolemmal and intermyofibrillar mitochondria

被引:84
作者
Servais, S
Couturier, K
Koubi, H
Rouanet, JL
Desplanches, D
Sornay-Mayet, MH
Sempore, B
Lavoie, JM
Favier, R
机构
[1] Univ Grenoble 1, Equipe Mixte INSERM 221, Lab Bioenerget Fondamentale & Appliquee, F-38041 Grenoble 9, France
[2] Univ Lyon 1, CNRS, UMR 5123, Lyon, France
[3] Univ Montreal, Dept Kinesiol, Quebec City, PQ, Canada
关键词
free wheel running; oxidative capacities; Lou/C rats; reactive oxygen species; heat shock protein; free radicals;
D O I
10.1016/S0891-5849(03)00177-1
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Previous data have demonstrated that, to handle the oxidative stress encountered with training at high intensity, skeletal muscle relies on an increase in mitochondrial biogenesis, a reduced H2O2 production, and an enhancement of antioxidant enzymes. In the present study, we evaluated the influence of voluntary running on mitochondrial O-2 consumption and H2O2 production by intermyofibrillar mitochondria (IFM) and subsarcolemmal mitochondria (SSM) isolated from oxidative muscles in conjunction with the determination of antioxidant capacities. When mitochondria are incubated with succinate as substrate, both maximal (state 3) and resting (state 4) O-2 consumption were significantly lower in SSM than in IFM populations. Mitochondrial H2O2 release per unit of O-2 consumed was 2-fold higher in SSM than in EFM. Inhibition of H2O2 formation by rotenone suggests that complex I of the electron transport chain is likely the major physiological H2O2-generating system. In Lou/C rats (an inbred strain of rats of Wistar origin), neither O-2 consumption nor H2O2 release by IFM and SSM were affected by long-term, voluntary wheel training. In contrast, glutathione peroxidase and catalase activity were significantly increased despite no change in oxidative capacities with long-term, voluntary exercise. Furthermore, chronic exercise enhanced heat shock protein 72 accumulation within skeletal muscle. It is concluded that the antioxidant status of muscle can be significantly improved. by prolonged wheel exercise without necessitating an increase in mitochondrial oxidative capacities. (C) 2003 Elsevier Inc.
引用
收藏
页码:24 / 32
页数:9
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