Protein Adsorption on a Glycosylated Polyacrylonitrile Surface: Monitoring with QCM and SPR

被引:15
作者
Che, Ai-Fu [1 ]
Huang, Xiao-Jun [1 ]
Xu, Zhi-Kang [1 ]
机构
[1] Zhejiang Univ, MOE Key Lab Macromol Synth & Functionalizat, Dept Polymer Sci & Engn, Hangzhou 310027, Peoples R China
基金
中国国家自然科学基金;
关键词
carbohydrates; Concanavalin A; glycosylated surfaces; molecular recognition; proteins; QUARTZ-CRYSTAL MICROBALANCE; MICROPOROUS POLYPROPYLENE MEMBRANES; INDUCED GRAFT-POLYMERIZATION; AIR-WATER-INTERFACE; CONCANAVALIN-A; PLASMON RESONANCE; CARBOHYDRATE INTERACTIONS; RECOGNITION; BINDING; FABRICATION;
D O I
10.1002/mabi.201000002
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A simple and efficient method to fabricate a glycosylated surface on a polyacrylonitrile-based film is described. Construction and protein adsorption processes were monitored in situ using a QCM. A PANCHEMA film was deposited on the gold surface of the quartz crystal, and the glycosylated surface was then constructed through surface modification. Con A and BSA were used as probes to study the specificity of this surface to proteins. It can recognize Con A, while showing no specific interaction with BSA. The binding affinity indicates the presence of strong multivalent interactions between Con A and the glucose residues (cluster glycoside effect). Reproducibility and repeatability of the glycosylated polymer surface are sufficient to allow specific adsorption of Con A.
引用
收藏
页码:955 / 962
页数:8
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