Differential Mechanism of Action of 3,4′,7-O-trimethylquercetin in Three Types of Ovarian Cancer Cells

被引：9

作者：

Ashraf, A. H. M. Zuberi ^{[1
]}

Afroze, Syeda H. ^{[2
]}

Yamauchi, Kosei ^{[3
]}

Zawieja, David C. ^{[2
]}

Keuhl, Thomas J. ^{[4
]}

Erlandson, Laura Weiser ^{[1
]}

Uddin, Mohammad N. ^{[3
,5
]}

机构：

[1] Texas A&M Univ Cent Texas, Killeen, TX USA

[2] Gifu Univ, Gifu, Japan

[3] Texas A&M Univ, Coll Med, Temple, TX 76508 USA

[4] Baylor Scott & White Hlth, Temple, TX USA

[5] Orion Inst Translat Med, Temple, TX 76504 USA

来源：

ANTICANCER RESEARCH | 2018年 / 38卷 / 09期

关键词：

Ovarian cancer; apoptosis; Annexin-V; methylquercetin; BAX/BCL-2; RATIO; QUERCETIN; MIGRATION; CYTOTROPHOBLAST; PATHOGENESIS; EXPRESSION;

D O I：

10.21873/anticanres.12835

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Background/Aim: 3,4',7-O-trimethylquercetin (34'7TMQ), a derivative of quercetin, inhibited ovarian cancer cell migration and invasion without affecting proliferation. In this study, the apoptotic effect of 34'7TMQ on three cancer cell lines (CRL-1978, CRL-11731, SK-OV-3) was evaluated. Materials and Methods: Expression of proapoptotic proteins such as Bax/Bcl-2 ratio, p38 MAP kinase, and caspase-9 were measured by western blot analysis. Annexin-V staining was performed to visualize apoptotic signaling. Results: Caspase-9 was up-regulated in all three cell lines. Bax/Bcl-2 ratio was up-regulated in CRL-1978 and SK-OV-3 but down-regulated in CRL-11731. The p38 MAPK was down-regulated in CRL-1978, up-regulated in SK-OV-3, and had differential expression in CRL-11731. Annexin V staining indicated that 34'7TMQ at 6.25 mu M induced apoptotic signaling in the CRL-1978 ovarian cancer cell line. Conclusion: 34'7TMQ induced apoptosis in three types of cancer cell lines but it appears to have a different mechanism of action in each cell line.

引用

页码：5131 / 5137

页数：7

共 25 条

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