Coupling development and elution, a new thin-layer chromatography technique

被引：4

作者：

Han, J ^{[1
]}

Wang, DY ^{[1
]}

Wang, D ^{[1
]}

Wang, YP ^{[1
]}

Zhou, M ^{[1
]}

Li, LD ^{[1
]}

Zhang, HX ^{[1
]}

机构：

[1] Shenyang Pharmaceut Univ, Dept Analyt Chem, Shenyang 110016, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY A | 2003年 / 1002卷 / 1-2期

关键词：

instrumentation; thin-layer chromatography;

D O I：

10.1016/S0021-9673(03)00686-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Three methods of coupling development and elution were studied in this paper. (1) A new mode of solvent supplementation and eluate collection was developed for descending development. By using a new distributor and collector in descending development, components can be separated and eluted continuously. (2) The same effect can be realized with a slope distributor [Su et al., J. Planar Chromatogr. 14 (2001) 203] and a collector by horizontal development. (3) In-situ elution can be used to treat a developed silica plate, which can elute the separated components to the receptor without scraping them off. These three methods can be used individually, and the in-situ elution can be used with other modes of development. (C) 2003 Elsevier Science B.V. All fights reserved.

引用

页码：213 / 219

页数：7

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