Enhanced osteogenic differentiation of stem cells by 3D printed PCL scaffolds coated with collagen and hydroxyapatite

被引:76
作者
Ebrahimi, Zahra [1 ]
Irani, Shiva [1 ]
Ardeshirylajimi, Abdolreza [2 ]
Seyedjafari, Ehsan [3 ]
机构
[1] Islamic Azad Univ, Dept Biol, Sci & Res Branch, Tehran, Iran
[2] Shahid Beheshti Univ Med Sci, Urogenital Stem Cell Res Ctr, Tehran, Iran
[3] Univ Tehran, Coll Sci, Dept Biotechnol, Tehran, Iran
基金
英国科研创新办公室;
关键词
COMPOSITE SCAFFOLDS; IN-VITRO; CALCIUM-PHOSPHATE; BONE; BIOMATERIALS; DEGRADATION; HYDROGELS; POLYMERS;
D O I
10.1038/s41598-022-15602-y
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Bone tissue engineering uses various methods and materials to find suitable scaffolds that regenerate lost bone due to disease or injury. Poly(epsilon-caprolactone) (PCL) can be used in 3D printing for producing biodegradable scaffolds by fused deposition modeling (FDM). However, the hydrophobic surfaces of PCL and its non-osteogenic nature reduces adhesion and cell bioactivity at the time of implantation. This work aims to enhance bone formation, osteogenic differentiation, and in vitro biocompatibility via PCL scaffolds modification with Hydroxyapatite (HA) and Collagen type I (COL). This study evaluated the osteosupportive capacity, biological behavior, and physicochemical properties of 3D-printed PCL, PCL/HA, PCL/COL, and PCL/HA/COL scaffolds. Biocompatibility and cells proliferation were investigated by seeding human adipose tissue-derived mesenchymal stem cells (hADSCs) onto the scaffolds, which were analyzed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, and 6-diamidino-2-phenylindole (DAPI) staining. In addition, the bone differentiation potential of the hADSCs was assessed using calcium deposition, alkaline phosphatase (ALP) activity, and bone-related protein and genes. Although all constructed scaffolds support hADSCs proliferation and differentiation, the results showed that scaffold coating with HA and COL can boost these capacities in a synergistic manner. According to the findings, the tricomponent 3D-printed scaffold can be considered as a promising choice for bone tissue regeneration and rebuilding.
引用
收藏
页数:15
相关论文
共 69 条
[1]   Graphene oxide incorporated polycaprolactone/chitosan/collagen electrospun scaffold: Enhanced osteogenic properties for bone tissue engineering [J].
Aidun, Amir ;
Firoozabady, Alireza Safaei ;
Moharrami, Mohammad ;
Ahmadi, Ali ;
Haghighipour, Nooshin ;
Bonakdar, Shahin ;
Faghihi, Shahab .
ARTIFICIAL ORGANS, 2019, 43 (10) :E264-E281
[2]   In vitro comparison of 3D printed polylactic acid/hydroxyapatite and polylactic acid/bioglass composite scaffolds: Insights into materials for bone regeneration [J].
Alksne, Milda ;
Kalvaityte, Migle ;
Simoliunas, Egidijus ;
Rinkunaite, Ieva ;
Gendviliene, Ieva ;
Locs, Janis ;
Rutkunas, Vygandas ;
Bukelskiene, Virginija .
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS, 2020, 104
[3]   Immobilization of carboxymethyl chitosan/laponite on polycaprolactone nanofibers as osteoinductive bone scaffolds [J].
Arab-Ahmadi, Samira ;
Irani, Shiva ;
Bakhshi, Hadi ;
Atyabi, Fatemeh ;
Ghalandari, Behafarid .
POLYMERS FOR ADVANCED TECHNOLOGIES, 2021, 32 (02) :755-765
[4]   3D printed polymer-mineral composite biomaterials for bone tissue engineering: Fabrication and characterization [J].
Babilotte, Joanna ;
Guduric, Vera ;
Le Nihouannen, Damien ;
Naveau, Adrien ;
Fricain, Jean-Christophe ;
Catros, Sylvain .
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, 2019, 107 (08) :2579-2595
[5]   Regeneration of Bone Defects in a Rabbit Femoral Osteonecrosis Model Using 3D-Printed Poly (Epsilon-Caprolactone)/Nanoparticulate Willemite Composite Scaffolds [J].
Bardeei, Latifeh Karimzadeh ;
Seyedjafari, Ehsan ;
Hossein, Ghamartaj ;
Nabiuni, Mohammad ;
Ara, Mohammad Hosein Majles ;
Salber, Jochen .
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2021, 22 (19)
[6]   Strategies for Bone Regeneration: From Graft to Tissue Engineering [J].
Battafarano, Giulia ;
Rossi, Michela ;
De Martino, Viviana ;
Marampon, Francesco ;
Borro, Luca ;
Secinaro, Aurelio ;
Del Fattore, Andrea .
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2021, 22 (03) :1-22
[7]   Boron Nitride Based Nanobiocomposites: Design by 3D Printing for Bone Tissue Engineering [J].
Belaid, Habib ;
Nagarajan, Sakthivel ;
Barou, Carole ;
Huon, Vincent ;
Bares, Jonathan ;
Balme, Sebastien ;
Miele, Philippe ;
Cornu, David ;
Cavailles, Vincent ;
Teyssier, Catherine ;
Bechelany, Mikhael .
ACS APPLIED BIO MATERIALS, 2020, 3 (04) :1865-1874
[8]   Effects of PCL, PEG and PLGA polymers on curcumin release from calcium phosphate matrix for in vitro and in vivo bone regeneration [J].
Bose, Susmita ;
Sarkar, Naboneeta ;
Banerjee, Dishary .
MATERIALS TODAY CHEMISTRY, 2018, 8 :110-120
[9]   Hydrolytic and oxidative degradation of electrospun supramolecular biomaterials: In vitro degradation pathways [J].
Brugmans, M. C. P. ;
Sontjens, S. H. M. ;
Cox, M. A. J. ;
Nandakumar, A. ;
Bosman, A. W. ;
Mes, T. ;
Janssen, H. M. ;
Bouten, C. V. C. ;
Baaijens, F. P. T. ;
Driessen-Mol, A. .
ACTA BIOMATERIALIA, 2015, 27 :21-31
[10]   3D printing of PCL/nano-hydroxyapatite scaffolds derived from biogenic sources for bone tissue engineering [J].
Cestari, Francesca ;
Petretta, Mauro ;
Yang, Yuejiao ;
Motta, Antonella ;
Grigolo, Brunella ;
Sglavo, Vincenzo M. .
SUSTAINABLE MATERIALS AND TECHNOLOGIES, 2021, 29