Genetic diversity of the mangaba GeneBank using microsatellites

被引：2

作者：

Silva, A. V. C. ^{[1
]}

Vitoria, M. F. ^{[2
]}

Azevedo, V. R. R. ^{[3
]}

Sa, A. J. ^{[4
]}

Naseimento, A. L. S. ^{[2
]}

Cardoso, M. N. ^{[2
]}

Soares, A. N. R. ^{[2
]}

Silva Junior, J. F. ^{[1
]}

Sledo, A. ^{[1
]}

机构：

[1] Embrapa Tabuleiros Costeiros, Aracaju, SE, Brazil

[2] Univ Fed Sergipe, Programa Posgrad Agr & Biodiversidade, Sao Cristovao, SE, Brazil

[3] Embrapa Recursos Genet, Brasilia, DF, Brazil

[4] Inst Fed Sergipe, Aracaju, SE, Brazil

来源：

GENETICS AND MOLECULAR RESEARCH | 2019年 / 18卷 / 01期

关键词：

Hancornia speciosa; Variability; Conservation; SSR; Brazilian fruit; Germplasm; HANCORNIA-SPECIOSA GOMES; NATURAL-POPULATIONS; DIFFERENTIATION;

D O I：

10.4238/gmr18108

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The mangaba, Hancornia speciosa, (Apocynaceae) is a fruit tree native to Brazil with predominantly extractivist production. The fruit can be consumed in natura; however, it is widely consumed as frozen pulp and ice cream produced by agroindustry companies. We evaluated the genetic diversity of 213 individuals that make up the mangaba GeneBank of Embrapa Tabuleiros Costeiros, using nine microsatellite markers (SSR). A total of 147 alleles were identified, with a mean of 16 alleles per locus; 100% polymorphism was observed among accessions. Reliability of the result was verified based on stress (0.042) and correlation (0.988) values. The alleles presented a high frequency of heterozygosity (He > Ho). The F-st (0.22) and f (0.07) values indicated moderate population structure, with great diversity within accessions. Bayesian analysis indicated the most adequate grouping with k = 2. The Unweighted Pair Group Method analysis showed three distinct groups according to similarity. The BI accession had the best genetic structure. The PM5/GX2, CN1/CN9, G18/PA1, JA14/JA15, and OI8/OI9 pairs of individuals are the closest genetically. We conclude that the Mangaba GeneBank has high diversity; this knowledge is relevant to develop strategies for the management of these genetic resources.

引用

页数：10

共 26 条

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