Suppression of nitric oxide production on LPS/IFN-γ-stimulated RAW264.7 macrophages by a novel catechin, pilosanol N, from Agrimonia pilosa Ledeb

被引：15

作者：

Taira, Junsei ^{[1
]}

Ohmine, Wakana ^{[1
]}

Ogi, Takayuki ^{[2
]}

Nanbu, Hitoshi ^{[3
]}

Ueda, Katsuhiro ^{[3
]}

机构：

[1] Okinawa Natl Coll Technol, Dept Bioresource Technol, Nago City, Okinawa 9052192, Japan

[2] Okinawa Ind Technol Ctr, Uruma City, Okinawa 9042234, Japan

[3] Univ Ryukyus, Dept Chem Biol & Marine Sci, Okinawa 9030213, Japan

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2012年 / 22卷 / 04期

关键词：

Nitric oxide; iNOS; Nitric oxide scavenger; Catechin; RAW264.7; macrophages; Agrimonia pilosa Ledeb; INHIBITION; SYNTHASE; PEROXYNITRITE; FLAVONOIDS; WOGONIN; INOS;

D O I：

10.1016/j.bmcl.2011.12.086

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

A novel catechin, pilosanol N (1), was isolated from Agrimonia pilosa Ledeb and its structure was determined by H-1, C-13 NMR and HRESI-MS analyses. Compound 1 inhibited nitric oxide (NO) production in LPS/IFN-gamma-induced RAW264.7 macrophages, and then the iNOS gene expression and its protein production with LPS/IFN-gamma treatment cells were also suppressed in the presence of 1. In addition, compound 1 scavenged NO or nitrogen radicals generated from NOR3 (4-ethyl-2-hydroxyamino-5-nitro-3-hexenamide) as an NO donor. These results indicated that pilosanol N can decrease the level of NO through a mechanism that involved both a decrease in the NO production and NO scavenging. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：1766 / 1769

页数：4

共 25 条

[1]

Akaike T., 2000, Nitric Oxide, P733

[2] The chemistry of DNA damage from nitric oxide and peroxynitrite [J].

Burney, S ;

Caulfield, JL ;

Niles, JC ;

Wishnok, JS ;

Tannenbaum, SR .

MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS, 1999, 424 (1-2) :37-49

[3] Enteric bacteria, lipopolysaccharides and related cytokines in inflammatory bowel disease: biological and clinical significance [J].

Caradonna, L ;

Amati, L ;

Magrone, T ;

Pellegrino, NM ;

Jirillo, E ;

Caccavo, D .

JOURNAL OF ENDOTOXIN RESEARCH, 2000, 6 (03) :205-214

[4] Wogonin, baicalin, and baicalein inhibition of inducible nitric oxide synthase and cyclooxygenase-2 gene expressions induced by nitric oxide synthase inhibitors and lipopolysaccharide [J].

Chen, YC ;

Shen, SC ;

Chen, LG ;

Lee, TJF ;

Yang, LL .

BIOCHEMICAL PHARMACOLOGY, 2001, 61 (11) :1417-1427

[5] Oroxylin A inhibition of lipopolysaccharide-induced iNOS and COX 2 gene expression via suppression of nuclear factor-κB activation [J].

Chen, YC ;

Yang, LL ;

Lee, TJF .

BIOCHEMICAL PHARMACOLOGY, 2000, 59 (11) :1445-1457

[6] MTT COLORIMETRIC ASSAY FOR TESTING MACROPHAGE CYTOTOXIC ACTIVITY INVITRO [J].

FERRARI, M ;

FORNASIERO, MC ;

ISETTA, AM .

JOURNAL OF IMMUNOLOGICAL METHODS, 1990, 131 (02) :165-172

[7] Peroxynitrite scavenging by flavonoids [J].

Haenen, GRMM ;

Paquay, JBG ;

Korthouwer, REM ;

Bast, A .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1997, 236 (03) :591-593

[8] Inhibition of nitric oxide synthase as a potential therapeutic target [J].

Hobbs, AJ ;

Higgs, A ;

Moncada, S .

ANNUAL REVIEW OF PHARMACOLOGY AND TOXICOLOGY, 1999, 39 :191-220

[9] Inhibitory Effect of Agrimonia pilosa Ledeb. on Inflammation by Suppression of iNOS and ROS Production [J].

Jung, Chang Hwa ;

Kim, Jeong-Hyun ;

Park, Sunju ;

Kweon, Dae-Hyuk ;

Kim, Sung-Hoon ;

Ko, Seong-Gyu .

IMMUNOLOGICAL INVESTIGATIONS, 2010, 39 (02) :159-170

[10] Acetylcholinesterase inhibition by flavonoids from agrimonia pilosa [J].

Jung, Mankil ;

Park, Moonsoo .

MOLECULES, 2007, 12 (09) :2130-2139

← 1 2 3 →