Ultrasensitive detection of SARS-CoV-2 nucleocapsid protein using large gold nanoparticle-enhanced surface plasmon resonance

被引：72

作者：

Yano, Taka-aki ^{[1
]}

Kajisa, Taira ^{[1
,7
]}

Ono, Masayuki ^{[2
]}

Miyasaka, Yoshiya ^{[2
]}

Hasegawa, Yuichi ^{[2
]}

Saito, Atsushi ^{[2
]}

Otsuka, Kunihiro ^{[3
]}

Sakane, Ayuko ^{[1
,4
]}

Sasaki, Takuya ^{[4
]}

Yasutomo, Koji ^{[1
,3
]}

Hamajima, Rina ^{[5
,8
]}

Kanai, Yuta ^{[5
]}

Kobayashi, Takeshi ^{[5
]}

Matsuura, Yoshiharu ^{[5
,6
]}

Itonaga, Makoto ^{[2
]}

Yasui, Takeshi ^{[1
]}

机构：

[1] Tokushima Univ, Inst Post LED Photon, 2-1 Minami Josanjima, Tokushima 7708506, Japan

[2] JVCKENWOOD Corp, 58-7 Shinmei Cho, Yokosuka, Kanagawa 2398550, Japan

[3] Univ Tokushima, Grad Sch Med Sci, Dept Immunol & Parasitol, 3-18-15 Kuramoto, Tokushima 7708503, Japan

[4] Tokushima Univ, Grad Sch Med Sci, Dept Biochem, 3-18-15 Kuramoto, Tokushima 7708503, Japan

[5] Osaka Univ, Res Inst Microbial Dis, 3-1 Yamadaoka, Suita, Osaka 5650871, Japan

[6] Osaka Univ, Ctr Infect Dis Educ & Res, 3-1 Yamadaoka, Suita, Osaka 5650871, Japan

[7] Toyo Univ, Grad Sch Interdisciplinary New Sci, Kawagoe, Saitama 3508585, Japan

[8] Nagoya Univ, Grad Sch Bioagr Sci, Lab Sericulture & Entomoresources, Nagoya, Aichi 4648601, Japan

来源：

SCIENTIFIC REPORTS | 2022年 / 12卷 / 01期

关键词：

D O I：

10.1038/s41598-022-05036-x

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The COVID-19 pandemic has created urgent demand for rapid detection of the SARS-CoV-2 coronavirus. Herein, we report highly sensitive detection of SARS-CoV-2 nucleocapsid protein (N protein) using nanoparticle-enhanced surface plasmon resonance (SPR) techniques. A crucial plasmonic role in significantly enhancing the limit of detection (LOD) is revealed for exceptionally large gold nanoparticles (AuNPs) with diameters of hundreds of nm. SPR enhanced by these large nanoparticles lowered the LOD of SARS-CoV-2 N protein to 85 fM, resulting in the highest SPR detection sensitivity ever obtained for SARS-CoV-2 N protein.

引用

页数：8

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